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Title: Abiotic protein fragmentation by manganese oxide: Implications for a mechanism to supply soil biota with oligopeptides

Journal Article · · Environmental Science and Technology
 [1];  [2];  [1];  [1];  [1];  [3]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Oregon State Univ., Corvallis, OR (United States)
  3. Oregon State Univ., Corvallis, OR (United States); Institut fur Bodenlandschaftforschung, Leibniz Zentrum fur Agrarlandschaftsforschung (ZALF), Muncheberg (Germany)
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The ability of plants and microorganisms to take up organic nitrogen in the form of free amino acids and oligopeptides has received increasing attention over the last two decades, yet the mechanisms for the formation of such compounds in soil environments remain poorly understood. We used Nuclear Magnetic Resonance (NMR) and Electron Paramagnetic Resonance (EPR) spectroscopies to distinguish the reaction of a model protein with a pedogenic oxide (Birnessite, MnO2) from its response to a phyllosilicate (Kaolinite). Our data demonstrate that birnessite fragments the model protein while kaolinite does not, resulting in soluble peptides that would be available to soil biota and confirming the existence of an abiotic pathway for the formation of organic nitrogen compounds for direct uptake by plants and microorganisms. The absence of reduced Mn(II) in the solution suggests that birnessite acts as a catalyst rather than an oxidant in this reaction. NMR and EPR spectroscopies are shown to be valuable tools to observe these reactions and capture the extent of protein transformation together with the extent of mineral response.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC05-76RL01830; AC02-05CH11231
OSTI ID:
1254585
Alternate ID(s):
OSTI ID: 1580807
Report Number(s):
PNNL-SA-108407; 48183; 48364; KP1704020
Journal Information:
Environmental Science and Technology, Vol. 50, Issue 7; ISSN 0013-936X
Publisher:
American Chemical Society (ACS)Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 24 works
Citation information provided by
Web of Science

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Cited By (4)

Fe(II) reduction of pyrolusite (β-MnO2) and secondary mineral evolution journal December 2017
Minerals in the rhizosphere: overlooked mediators of soil nitrogen availability to plants and microbes journal June 2018
The laccase-like reactivity of manganese oxide nanomaterials for pollutant conversion: rate analysis and cyclic voltammetry journal August 2017
Carbohydrates protect protein against abiotic fragmentation by soil minerals journal January 2018

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59 BASIC BIOLOGICAL SCIENCES
Environmental Molecular Sciences Laboratory