Understanding dynamic changes in live cell adhesion with neutron reflectometry

Junghans, Ann; Waltman, Mary Jo; Smith, Hillary L.; Pocivavsek, Luka; Zebda, Noureddine; Birukov, Konstantin; Viapiano, Mariano; Majewski, Jaroslaw

doi:10.1142/S0217984914300154

Title: Understanding dynamic changes in live cell adhesion with neutron reflectometry

Journal Article · Wed Dec 10 00:00:00 EST 2014 · Modern Physics Letters B

DOI:https://doi.org/10.1142/S0217984914300154· OSTI ID:1246927

Junghans, Ann ^[1]; Waltman, Mary Jo ^[1]; Smith, Hillary L. ^[2]; Pocivavsek, Luka ^[3]; Zebda, Noureddine ^[4]; Birukov, Konstantin ^[5]; Viapiano, Mariano ^[6]; Majewski, Jaroslaw ^[1]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
California Institute of Technology, Pasadena, CA (United States)
Univ. of Pittsburgh Medical Center, Pittsburgh, PA (United States)
NDA Analytics, Cambridgeshire (United Kingdom)
Univ. of Chicago, Chicago, IL (United States)
Brigham and Women's Hospital and Harvard Medical School, Boston, MA (United States)

In this study, neutron reflectometry (NR) was used to examine various live cells' adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell — surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1246927

Report Number(s):: LA-UR-14-26647

Journal Information:: Modern Physics Letters B, Vol. 28, Issue 30; ISSN 0217-9849

Publisher:: World Scientific PublishingCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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59 BASIC BIOLOGICAL SCIENCES
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endothelial monolayer
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shear stress
neutron scattering

Title: Understanding dynamic changes in live cell adhesion with neutron reflectometry

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