In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells

Jakobi, Arjen J.; Passon, Daniel M.; Knoops, Kevin; Stellato, Francesco; Liang, Mengning; White, Thomas A.; Seine, Thomas; Messerschmidt, Marc; Chapman, Henry N.; Wilmanns, Matthias

doi:10.1107/S2052252515022927

Title: In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells

Journal Article · Tue Mar 01 00:00:00 EST 2016 · IUCrJ

DOI:https://doi.org/10.1107/S2052252515022927· OSTI ID:1244994

Jakobi, Arjen J. ^[1]; Passon, Daniel M. ^[2]; Knoops, Kevin ^[3]; Stellato, Francesco ^[2]; Liang, Mengning ^[2]; White, Thomas A. ^[2]; Seine, Thomas ^[2]; Messerschmidt, Marc ^[4]; Chapman, Henry N. ^[5]; Wilmanns, Matthias ^[6]

Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); European Molecular Biology Lab. (EMBL), Heidelberg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
Univ. of Groningen, Groningen (The Netherlands)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Center for Ultrafast Imaging, Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. Medical Center Hamburg-Eppendorf, Hamburg (Germany)

The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. Furthermore, the observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1244994

Alternate ID(s):: OSTI ID: 1307374

Report Number(s):: AC02-76SF00515; IUCRAJ; PII: S2052252515022927

Journal Information:: IUCrJ, Vol. 3, Issue 2; ISSN 2052-2525

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 19 works

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Title: In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells

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