PERTRAN: Genome-guided RNA-seq Read Assembler
Conference
·
OSTI ID:1241180
As short RNA-seq reads become a standard, affordable input to any genome annotation project, a sensitive and accurate transcript assembler is an essential part of any gene prediction system. PERTRAN is a pipeline for assembling transcripts from RNA-seq reads which demonstrates higher sensitivity, with fewer fused exons (in most cases), and faster run times compared to other TOPHAT/CUFFLINKS and genome-guided Trinity. PERTRAN shows slightly lower specificity with increased gene fusions in some cases, discussed below. SAM files generated from PERTRAN can be used to compute expression level by cuffdiff and result is comparable to that from TOPHAT.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 1241180
- Report Number(s):
- LBNL-7081E
- Resource Relation:
- Conference: Cold Spring Harbor Lab Genome Informatics, Cold Spring Harbor, New York, Oct 30 - Nov 2, 2013
- Country of Publication:
- United States
- Language:
- English
Similar Records
Rnnotator: an automated de novo transcriptome assembly pipeline from stranded RNA-Seq reads
Improving RNA-Seq Precision with MapAl
Defining the maize transcriptome de novo using deep RNA-Seq
Journal Article
·
Fri Nov 19 00:00:00 EST 2010
· BMC Genomics
·
OSTI ID:1241180
+6 more
Improving RNA-Seq Precision with MapAl
Journal Article
·
Mon Apr 02 00:00:00 EDT 2012
· Frontiers in Genetics
·
OSTI ID:1241180
+1 more
Defining the maize transcriptome de novo using deep RNA-Seq
Conference
·
Thu Jun 02 00:00:00 EDT 2011
·
OSTI ID:1241180
+4 more