Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta

Patil, Shrikant; Moeys, Sara; von Dassow, Peter; Huysman, Marie J. J.; Mapleson, Daniel; De Veylder, Lieven; Sanges, Remo; Vyverman, Wim; Montresor, Marina; Ferrante, Maria Immacolata

doi:10.1186/s12864-015-1983-5

Title: Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta

Journal Article · Sat Nov 14 00:00:00 EST 2015 · BMC Genomics

DOI:https://doi.org/10.1186/s12864-015-1983-5· OSTI ID:1241144

Patil, Shrikant ^[1]; Moeys, Sara ^[2]; von Dassow, Peter ^[3]; Huysman, Marie J. J. ^[2]; Mapleson, Daniel ^[4]; De Veylder, Lieven ^[2]; Sanges, Remo ^[1]; Vyverman, Wim ^[5]; Montresor, Marina ^[1]; Ferrante, Maria Immacolata ^[1]

Stazione Zoologica Anton Dohrn, Naples (Italy)
Ghent Univ., Ghent (Belgium); Flanders Institute for Biotechnology, Ghent (Belgium)
Pontificia Univ. Catolica de Chile, Santiago (Chile); Sorbonne Univ., Roscoff (France)
The Genome Analysis Centre (TGAC), Norwich (United Kingdom)
Ghent Univ., Ghent (Belgium)

Sexual reproduction is an obligate phase in the life cycle of most eukaryotes. Meiosis varies among organisms, which is reflected by the variability of the gene set associated to the process. Diatoms are unicellular organisms that belong to the stramenopile clade and have unique life cycles that can include a sexual phase. The exploration of five diatom genomes and one diatom transcriptome led to the identification of 42 genes potentially involved in meiosis. While these include the majority of known meiosis-related genes, several meiosis-specific genes, including DMC1, could not be identified. Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. The two sexual species Pseudo-nitzschia multistriata and Seminavis robusta were used to explore the expression of meiosis-related genes: RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. An almost identical toolkit is shared among Pseudo-nitzschia multiseries and Fragilariopsis cylindrus, as well as two species for which sex has not been observed, Phaeodactylum tricornutum and Thalassiosira pseudonana, suggesting that these two may retain a facultative sexual phase. Lastly, our results reveal the conserved meiotic toolkit in six diatom species and indicate that Stramenopiles share major modifications of canonical meiosis processes ancestral to eukaryotes, with important divergences in each Kingdom.

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Research Organization:: Univ. of California (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1241144

Journal Information:: BMC Genomics, Vol. 16, Issue 1; ISSN 1471-2164

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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MRP3 is a sex determining gene in the diatom Pseudo-nitzschia multistriata Russo, Monia T.; Vitale, Laura; Entrambasaguas, Laura Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-07496-0	journal	November 2018
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Putatively asexual chrysophytes have meiotic genes: evidence from transcriptomic data Kraus, Diana; Chi, Jingyun; Boenigk, Jens PeerJ, Vol. 6 https://doi.org/10.7717/peerj.5894	journal	January 2019
Correction to: Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter BMC Genomics, Vol. 20, Issue 1 https://doi.org/10.1186/s12864-019-5942-4	journal	July 2019
Morphological and transcriptomic evidence for ammonium induction of sexual reproduction in Thalassiosira pseudonana and other centric diatoms Moore, Eric R.; Bullington, Briana S.; Weisberg, Alexandra J. PLOS ONE https://doi.org/10.1101/144667	journal	June 2017
Finding a partner in the ocean: molecular and evolutionary bases of the response to sexual cues in a planktonic diatom Basu, Swaraj; Patil, Shrikant; Mapleson, Daniel New Phytologist, Vol. 215, Issue 1 https://doi.org/10.1111/nph.14557	journal	April 2017

Linked Research (26)

Figures / Tables (10)

Table 1(p. 3)

Table 1 (Continued)(p. 4)

Table 2(p. 5)

Table 2 (Continued)(p. 6)

Table 2 (Continued)(p. 7)

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
meiosis
diatoms
sexual reproduction
SPO11
RAD51

Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta [Supplemental Data] Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 10: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d10 The current record is supplemented by this dataset	dataset	January 2015
Additional file 6: Figure S19. of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d12 The current record is supplemented by this text	text	January 2015
Additional file 12: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d11 The current record is supplemented by this dataset	dataset	January 2015
Additional file 3: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d6.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 6: Figure S19. of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d12.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 5: Table S1. of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d1 The current record is supplemented by this text	text	January 2015
Additional file 1: Figures S1-S18. of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d2.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 11: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d5.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 4: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d8.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 3: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d6 The current record is supplemented by this text	text	January 2015
Additional file 9: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d7 The current record is supplemented by this dataset	dataset	January 2015
Additional file 4: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d8 The current record is supplemented by this text	text	January 2015
Additional file 8: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d9 The current record is supplemented by this dataset	dataset	January 2015
Additional file 1: Figures S1-S18. of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d2 The current record is supplemented by this text	text	January 2015
Additional file 7: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d3 The current record is supplemented by this text	text	January 2015
Additional file 2: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d4 The current record is supplemented by this text	text	January 2015
Additional file 11: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter Von https://doi.org/10.6084/m9.figshare.c.3613499_d5 The current record is supplemented by this text	text	January 2015
Additional file 10: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d10.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 9: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d7.v1 The current record is supplemented by this dataset	dataset	November 2015
Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta [Supplementary Data] Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499 The current record is supplemented by this dataset	dataset	November 2015
Additional file 8: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d9.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 12: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d11.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 5: Table S1. of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d1.v1 The current record is supplemented by this preprint	preprint	November 2015
Additional file 2: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; von Dassow, Peter https://doi.org/10.6084/m9.figshare.c.3613499_d4.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 7: of Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta Patil, Shrikant; Moeys, Sara; Dassow, Peter von https://doi.org/10.6084/m9.figshare.c.3613499_d3.v1 The current record is supplemented by this dataset	dataset	November 2015