Crystal structure of phototoxic orange fluorescent proteins with α tryptophan-based chromophore

Pletneva, Nadya V.; Pletnev, Vladimir Z.; Sarkisyan, Karen S.; Gorbachev, Dmitry A.; Egorov, Evgeny S.; Mishin, Alexander S.; Lukyanov, Konstantin A.; Dauter, Zbigniew; Pletnev, Sergei

doi:10.1371/journal.pone.0145740

Title: Crystal structure of phototoxic orange fluorescent proteins with α tryptophan-based chromophore

Journal Article · Wed Dec 23 00:00:00 EST 2015 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0145740· OSTI ID:1239529

Pletneva, Nadya V. ^[1]; Pletnev, Vladimir Z. ^[1]; Sarkisyan, Karen S. ^[1]; Gorbachev, Dmitry A. ^[2]; Egorov, Evgeny S. ^[1]; Mishin, Alexander S. ^[3]; Lukyanov, Konstantin A. ^[3]; Dauter, Zbigniew ^[4]; Pletnev, Sergei ^[5]

Russian Academy of Sciences (RAS), Moscow (Russian Federation)
Russian Academy of Sciences (RAS), Moscow (Russian Federation); Moscow State Univ., Moscow (Russian Federation)
Russian Academy of Sciences (RAS), Moscow (Russian Federation); Nizhny Novgorod State Medical Academy, Nizhny Novgorod (Russian Federation)
National Cancer Insitute, Argonne, IL (United States)
National Cancer Insitute, Argonne, IL (United States); Leidos Biomedical Research, Inc., Argonne, IL (United States)

Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. We present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKiller-Orange, at 1.81 and 1.57 Å resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the β-barrel exterior and enabling transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI ID:: 1239529

Journal Information:: PLoS ONE, Vol. 10, Issue 12; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 20 works

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A proton transfer network that generates deprotonated tyrosine is a key to producing reactive oxygen species in phototoxic KillerRed protein Lee, Wook; Kim, Inkoo; Rhee, Young Min Physical Chemistry Chemical Physics, Vol. 20, Issue 34 https://doi.org/10.1039/c8cp02939c	journal	January 2018
Plasmonic photocatalyst-like fluorescent proteins for generating reactive oxygen species Leem, Jung Woo; Kim, Seong-Ryul; Choi, Kwang-Ho Nano Convergence, Vol. 5, Issue 1 https://doi.org/10.1186/s40580-018-0140-7	journal	March 2018
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