Tracking single-particle rotation during macrophage uptake
Abstract
We investigated the rotational dynamics of single microparticles during their internalization by macrophage cells. The microparticles used were triblock patchy particles that display two fluorescent patches on their two poles. The optical anisotropy made it possible to directly visualize and quantify the orientation and rotation of the particles. We show that particles exhibit a mixture of fast and slow rotation as they are uptaken by macrophages and transiently undergo directional rotation during their entry into the cell. As a result, the size of the particles and the surface presentation of ligands exerted a negligible influence on this heterogeneity of particle rotation.
- Authors:
-
- Indiana Univ., Bloomington, IN (United States)
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- Indiana Univ., Bloomington, IN (United States)
- OSTI Identifier:
- 1235366
- Report Number(s):
- SAND-2015-3290J
Journal ID: ISSN 1744-683X; SMOABF; 583488
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Soft Matter
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 26; Journal ID: ISSN 1744-683X
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Sanchez, Lucero, Patton, Paul, Anthony, Stephen Michael, Yi, Yi, and Yu, Yan. Tracking single-particle rotation during macrophage uptake. United States: N. p., 2015.
Web. doi:10.1039/C5SM00893J.
Sanchez, Lucero, Patton, Paul, Anthony, Stephen Michael, Yi, Yi, & Yu, Yan. Tracking single-particle rotation during macrophage uptake. United States. https://doi.org/10.1039/C5SM00893J
Sanchez, Lucero, Patton, Paul, Anthony, Stephen Michael, Yi, Yi, and Yu, Yan. 2015.
"Tracking single-particle rotation during macrophage uptake". United States. https://doi.org/10.1039/C5SM00893J. https://www.osti.gov/servlets/purl/1235366.
@article{osti_1235366,
title = {Tracking single-particle rotation during macrophage uptake},
author = {Sanchez, Lucero and Patton, Paul and Anthony, Stephen Michael and Yi, Yi and Yu, Yan},
abstractNote = {We investigated the rotational dynamics of single microparticles during their internalization by macrophage cells. The microparticles used were triblock patchy particles that display two fluorescent patches on their two poles. The optical anisotropy made it possible to directly visualize and quantify the orientation and rotation of the particles. We show that particles exhibit a mixture of fast and slow rotation as they are uptaken by macrophages and transiently undergo directional rotation during their entry into the cell. As a result, the size of the particles and the surface presentation of ligands exerted a negligible influence on this heterogeneity of particle rotation.},
doi = {10.1039/C5SM00893J},
url = {https://www.osti.gov/biblio/1235366},
journal = {Soft Matter},
issn = {1744-683X},
number = 26,
volume = 11,
place = {United States},
year = {Wed Jun 10 00:00:00 EDT 2015},
month = {Wed Jun 10 00:00:00 EDT 2015}
}
Web of Science
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