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Title: Tracking single-particle rotation during macrophage uptake

Abstract

We investigated the rotational dynamics of single microparticles during their internalization by macrophage cells. The microparticles used were triblock patchy particles that display two fluorescent patches on their two poles. The optical anisotropy made it possible to directly visualize and quantify the orientation and rotation of the particles. We show that particles exhibit a mixture of fast and slow rotation as they are uptaken by macrophages and transiently undergo directional rotation during their entry into the cell. As a result, the size of the particles and the surface presentation of ligands exerted a negligible influence on this heterogeneity of particle rotation.

Authors:
 [1];  [1];  [2];  [1];  [1]
  1. Indiana Univ., Bloomington, IN (United States)
  2. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
Indiana Univ., Bloomington, IN (United States)
OSTI Identifier:
1235366
Report Number(s):
SAND-2015-3290J
Journal ID: ISSN 1744-683X; SMOABF; 583488
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Soft Matter
Additional Journal Information:
Journal Volume: 11; Journal Issue: 26; Journal ID: ISSN 1744-683X
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Sanchez, Lucero, Patton, Paul, Anthony, Stephen Michael, Yi, Yi, and Yu, Yan. Tracking single-particle rotation during macrophage uptake. United States: N. p., 2015. Web. doi:10.1039/C5SM00893J.
Sanchez, Lucero, Patton, Paul, Anthony, Stephen Michael, Yi, Yi, & Yu, Yan. Tracking single-particle rotation during macrophage uptake. United States. https://doi.org/10.1039/C5SM00893J
Sanchez, Lucero, Patton, Paul, Anthony, Stephen Michael, Yi, Yi, and Yu, Yan. 2015. "Tracking single-particle rotation during macrophage uptake". United States. https://doi.org/10.1039/C5SM00893J. https://www.osti.gov/servlets/purl/1235366.
@article{osti_1235366,
title = {Tracking single-particle rotation during macrophage uptake},
author = {Sanchez, Lucero and Patton, Paul and Anthony, Stephen Michael and Yi, Yi and Yu, Yan},
abstractNote = {We investigated the rotational dynamics of single microparticles during their internalization by macrophage cells. The microparticles used were triblock patchy particles that display two fluorescent patches on their two poles. The optical anisotropy made it possible to directly visualize and quantify the orientation and rotation of the particles. We show that particles exhibit a mixture of fast and slow rotation as they are uptaken by macrophages and transiently undergo directional rotation during their entry into the cell. As a result, the size of the particles and the surface presentation of ligands exerted a negligible influence on this heterogeneity of particle rotation.},
doi = {10.1039/C5SM00893J},
url = {https://www.osti.gov/biblio/1235366}, journal = {Soft Matter},
issn = {1744-683X},
number = 26,
volume = 11,
place = {United States},
year = {Wed Jun 10 00:00:00 EDT 2015},
month = {Wed Jun 10 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
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Citation Metrics:
Cited by: 23 works
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Works referenced in this record:

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Works referencing / citing this record:

Janus particles: from synthesis to application
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Janus particles for biological imaging and sensing
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Effect of partial PEGylation on particle uptake by macrophages
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Measuring rotational diffusion of colloidal spheres with confocal microscopy
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Generation of 3-dimensional multi-patches on silica particles via printing with wrinkled stamps
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Mono-patchy zwitterionic microcolloids as building blocks for pH-controlled self-assembly
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