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A limited role for carbonic anhydrase in C4 photosynthesis as revealed by a ca1ca2 double mutant in maize.
Abstract
Carbonic anhydrase (CA) catalyzes the first biochemical step of the carbon concentrating mechanism of C4 plants, and in C4 monocots, it has been suggested that CA activity is near limiting for photosynthesis. Here, we test this hypothesis through the characterization of transposon induced mutant alleles of Ca1 and Ca2 in Zea mays. In addition, these two isoforms account for more than 85% of the CA transcript pool. A significant change in isotopic discrimination is observed in mutant plants, which have as little as 3% of wild-type CA activity, but surprisingly, photosynthesis is not reduced under current or elevated pCO2. However, growth and rates of photosynthesis under sub-ambient pCO2 are significantly impaired in the mutants. These findings suggest, that while CA is not limiting for C4 photosynthesis in Z. mays at current pCO2, it likely maintains high rates of photosynthesis when CO2 availability is reduced. Current atmospheric CO2 levels now exceed 400 ppm (~40.53 Pa) and contrast the low CO2 partial pressure (pCO2) conditions under which C4 plants expanded their range ~10 million years ago when the global atmospheric CO2 was below 300 ppm (~30.40 Pa). Thus, as CO2 levels continue to rise, selective pressures for high levels of CA maymore »
- Authors:
-
- Donald Danforth Plant Science Center, St. Louis, MO (United States)
- Washington State Univ., Pullman, WA (United States)
- Publication Date:
- Research Org.:
- Life Sciences Research Foundation, Baltimore, MD (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- Contributing Org.:
- Life Sciences Research Foundation
- OSTI Identifier:
- 1233449
- Grant/Contract Number:
- SC0008510
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Volume: 165; Journal Issue: 2; Journal ID: ISSN 0032-0889
- Publisher:
- American Society of Plant Biologists
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Studer, Anthony J., Gandin, Anthony, Kolbe, Allison R., Wang, Lin, Cousins, Asaph B., and Brutnell, Thomas P. A limited role for carbonic anhydrase in C4 photosynthesis as revealed by a ca1ca2 double mutant in maize.. United States: N. p., 2014.
Web. doi:10.1104/pp.114.237602.
Studer, Anthony J., Gandin, Anthony, Kolbe, Allison R., Wang, Lin, Cousins, Asaph B., & Brutnell, Thomas P. A limited role for carbonic anhydrase in C4 photosynthesis as revealed by a ca1ca2 double mutant in maize.. United States. https://doi.org/10.1104/pp.114.237602
Studer, Anthony J., Gandin, Anthony, Kolbe, Allison R., Wang, Lin, Cousins, Asaph B., and Brutnell, Thomas P. 2014.
"A limited role for carbonic anhydrase in C4 photosynthesis as revealed by a ca1ca2 double mutant in maize.". United States. https://doi.org/10.1104/pp.114.237602. https://www.osti.gov/servlets/purl/1233449.
@article{osti_1233449,
title = {A limited role for carbonic anhydrase in C4 photosynthesis as revealed by a ca1ca2 double mutant in maize.},
author = {Studer, Anthony J. and Gandin, Anthony and Kolbe, Allison R. and Wang, Lin and Cousins, Asaph B. and Brutnell, Thomas P.},
abstractNote = {Carbonic anhydrase (CA) catalyzes the first biochemical step of the carbon concentrating mechanism of C4 plants, and in C4 monocots, it has been suggested that CA activity is near limiting for photosynthesis. Here, we test this hypothesis through the characterization of transposon induced mutant alleles of Ca1 and Ca2 in Zea mays. In addition, these two isoforms account for more than 85% of the CA transcript pool. A significant change in isotopic discrimination is observed in mutant plants, which have as little as 3% of wild-type CA activity, but surprisingly, photosynthesis is not reduced under current or elevated pCO2. However, growth and rates of photosynthesis under sub-ambient pCO2 are significantly impaired in the mutants. These findings suggest, that while CA is not limiting for C4 photosynthesis in Z. mays at current pCO2, it likely maintains high rates of photosynthesis when CO2 availability is reduced. Current atmospheric CO2 levels now exceed 400 ppm (~40.53 Pa) and contrast the low CO2 partial pressure (pCO2) conditions under which C4 plants expanded their range ~10 million years ago when the global atmospheric CO2 was below 300 ppm (~30.40 Pa). Thus, as CO2 levels continue to rise, selective pressures for high levels of CA may be limited to arid climates where stomatal closure reduces CO2 availability to the leaf.},
doi = {10.1104/pp.114.237602},
url = {https://www.osti.gov/biblio/1233449},
journal = {Plant Physiology (Bethesda)},
issn = {0032-0889},
number = 2,
volume = 165,
place = {United States},
year = {Fri Apr 04 00:00:00 EDT 2014},
month = {Fri Apr 04 00:00:00 EDT 2014}
}
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Figures / Tables:
Figure 1: Ds insertional mutants disrupt the two most highly expressed CA genes. (a) Unique RNA-seq reads from the tip of the 3rd leaf of a maize seedling were mapped to the 3’ UTR of each carbonic anhydrase gene to quantify the expression of each member of the gene familymore »
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