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Title: Multifunctional cellulase and hemicellulase

Patent ·
OSTI ID:1221980
; ;

A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.

Research Organization:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC02-07ER64494
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Number(s):
9,145,551
Application Number:
14/030,290
OSTI ID:
1221980
Resource Relation:
Patent File Date: 2013 Sep 18
Country of Publication:
United States
Language:
English

References (18)

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Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles journal September 2007
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A major new component in the cellulosome of Clostridium thermocellum is a processive endo-β-1,4-glucanase producing cellotetraose journal August 2005
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Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate journal July 2011
Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition journal January 2007
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A combined approach to improving large-scale production of tobacco etch virus protease journal September 2007
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Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio journal March 2011
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Related Subjects

59 BASIC BIOLOGICAL SCIENCES