Structural Divergence in Vertebrate Phylogeny of a Duplicated Prototype Galectin
Abstract
Prototype galectins, endogenously expressed animal lectins with a single carbohydrate recognition domain, are well-known regulators of tissue properties such as growth and adhesion. The earliest discovered and best studied of the prototype galectins is Galectin-1 (Gal-1). In the Gallus gallus (chicken) genome, Gal-1 is represented by two homologs: Gal-1A and Gal-1B, with distinct biochemical properties, tissue expression, and developmental functions. We investigated the origin of the Gal-1A/Gal-1B divergence to gain insight into when their developmental functions originated and how they could have contributed to vertebrate phenotypic evolution. Sequence alignment and phylogenetic tree construction showed that the Gal-1A/Gal-1B divergence can be traced back to the origin of the sauropsid lineage (consisting of extinct and extant reptiles and birds) although lineage-specific duplications also occurred in the amphibian and actinopterygian genomes. Gene synteny analysis showed that sauropsid gal-1b (the gene for Gal-1B) and its frog and actinopterygian gal-1 homologs share a similar chromosomal location, whereas sauropsid gal-1a has translocated to a new position. Surprisingly, we found that chicken Gal-1A, encoded by the translocated gal-1a, was more similar in its tertiary folding pattern than Gal-1B, encoded by the untranslocated gal-1b, to experimentally determined and predicted folds of nonsauropsid Gal-1s. This inference is consistent withmore »
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States), Life Sciences Division
- Univ. of California, Irvine, CA (United States). Department of Ecology and Evolutionary Biology
- Univ. College London, (United Kingdom). Department of Computer Science
- New York Medical College, Valhalla, New York, (United States). Department of Cell Biology and Anatomy
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1214388
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Genome Biology and Evolution
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 10; Journal ID: ISSN 1759-6653
- Publisher:
- Society for Molecular Biology and Evolution
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; prototype galectin; galectin-1; sauropsids; protein fold; homology
Citation Formats
Bhat, R., Chakraborty, M., Mian, I. S., and Newman, S. A. Structural Divergence in Vertebrate Phylogeny of a Duplicated Prototype Galectin. United States: N. p., 2014.
Web. doi:10.1093/gbe/evu215.
Bhat, R., Chakraborty, M., Mian, I. S., & Newman, S. A. Structural Divergence in Vertebrate Phylogeny of a Duplicated Prototype Galectin. United States. https://doi.org/10.1093/gbe/evu215
Bhat, R., Chakraborty, M., Mian, I. S., and Newman, S. A. 2014.
"Structural Divergence in Vertebrate Phylogeny of a Duplicated Prototype Galectin". United States. https://doi.org/10.1093/gbe/evu215. https://www.osti.gov/servlets/purl/1214388.
@article{osti_1214388,
title = {Structural Divergence in Vertebrate Phylogeny of a Duplicated Prototype Galectin},
author = {Bhat, R. and Chakraborty, M. and Mian, I. S. and Newman, S. A.},
abstractNote = {Prototype galectins, endogenously expressed animal lectins with a single carbohydrate recognition domain, are well-known regulators of tissue properties such as growth and adhesion. The earliest discovered and best studied of the prototype galectins is Galectin-1 (Gal-1). In the Gallus gallus (chicken) genome, Gal-1 is represented by two homologs: Gal-1A and Gal-1B, with distinct biochemical properties, tissue expression, and developmental functions. We investigated the origin of the Gal-1A/Gal-1B divergence to gain insight into when their developmental functions originated and how they could have contributed to vertebrate phenotypic evolution. Sequence alignment and phylogenetic tree construction showed that the Gal-1A/Gal-1B divergence can be traced back to the origin of the sauropsid lineage (consisting of extinct and extant reptiles and birds) although lineage-specific duplications also occurred in the amphibian and actinopterygian genomes. Gene synteny analysis showed that sauropsid gal-1b (the gene for Gal-1B) and its frog and actinopterygian gal-1 homologs share a similar chromosomal location, whereas sauropsid gal-1a has translocated to a new position. Surprisingly, we found that chicken Gal-1A, encoded by the translocated gal-1a, was more similar in its tertiary folding pattern than Gal-1B, encoded by the untranslocated gal-1b, to experimentally determined and predicted folds of nonsauropsid Gal-1s. This inference is consistent with our finding of a lower proportion of conserved residues in sauropsid Gal-1Bs, and evidence for positive selection of sauropsid gal-1b, but not gal-1a genes. We propose that the duplication and structural divergence of Gal-1B away from Gal-1A led to specialization in both expression and function in the sauropsid lineage.},
doi = {10.1093/gbe/evu215},
url = {https://www.osti.gov/biblio/1214388},
journal = {Genome Biology and Evolution},
issn = {1759-6653},
number = 10,
volume = 6,
place = {United States},
year = {Thu Sep 25 00:00:00 EDT 2014},
month = {Thu Sep 25 00:00:00 EDT 2014}
}
Web of Science
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