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Title: An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis

Journal Article · · BMC Microbiology
 [1];  [1];  [2];  [3];  [4];  [1]
  1. Chinese Academy of Sciences, Wuhan (China); Univ. of Chinese Academy of Sciences, Beijing (China)
  2. Hubei Univ. of Engineering, Xiaogan (China)
  3. Univ. of Virginia, Charlottesville, VA (United States)
  4. Univ. of Oklahoma, Norman, OK (United States)
+ Show Author Affiliations

Background: Bacteria use alternative sigma factors (σs) to regulate condition-specific gene expression for survival and Shewanella harbors multiple ECF (extracytoplasmic function) σ genes and cognate anti-sigma factor genes. Here we comparatively analyzed two of the rpoE-like operons in the strain MR-1: rpoE-rseA-rseB-rseC and rpoE2-chrR. Results: RpoE was important for bacterial growth at low and high temperatures, in the minimal medium, and high salinity. The degP/htrA orthologue, required for growth of Escherichia coli and Pseudomonas aeruginosa at high temperature, is absent in Shewanella, while the degQ gene is RpoE-regulated and is required for bacterial growth at high temperature. RpoE2 was essential for the optimal growth in oxidative stress conditions because the rpoE2 mutant was sensitive to hydrogen peroxide and paraquat. The operon encoding a ferrochelatase paralogue (HemH2) and a periplasmic glutathione peroxidase (PgpD) was identified as RpoE2-dependent. PgpD exhibited higher activities and played a more important role in the oxidative stress responses than the cytoplasmic glutathione peroxidase CgpD under tested conditions. The rpoE2-chrR operon and the identified regulon genes, including pgpD and hemH2, are coincidently absent in several psychrophilic and/or deep-sea Shewanella strains. Conclusion: In S. oneidensis MR-1, the RpoE-dependent degQ gene is required for optimal growth under high temperature. The rpoE2 and RpoE2-dependent pgpD gene encoding a periplasmic glutathione peroxidase are involved in oxidative stress responses. But rpoE2 is not required for bacterial growth at low temperature and it even affected bacterial growth under salt stress, indicating that there is a tradeoff between the salt resistance and RpoE2-mediated oxidative stress responses.

Research Organization:
Univ. of Oklahoma, Norman, OK (United States)
Sponsoring Organization:
USDOE; Chinese Academy of Science, Beijing (China)
Grant/Contract Number:
FG02-07ER64383; Y15103-1-401
OSTI ID:
1204353
Journal Information:
BMC Microbiology, Vol. 15, Issue 1; ISSN 1471-2180
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 25 works
Citation information provided by
Web of Science

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Cited By (7)

RpoN (σ54) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain journal May 2017
Both widespread PEP-CTERM proteins and exopolysaccharides are required for floc formation of Zoogloea resiniphila and other activated sludge bacteria: PEP-CTERM protein biomarker for floc formation journal March 2018
DNA phosphorothioate modification—a new multi-functional epigenetic system in bacteria journal October 2018
Extracellular reduction of solid electron acceptors by Shewanella oneidensis journal July 2018
Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins journal July 2019
Extracellular reduction of solid electron acceptors by Shewanella oneidensis text January 2018
Extracellular reduction of solid electron acceptors by Shewanella oneidensis text January 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Periplasmic glutathione peroxidase
Shewanella
ECF sigma factor
Oxidative stress response