Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs
Abstract
Staphylococcus aureus infection is not associated with the development of protective immunity, and disease relapses occur frequently. We hypothesize that protein A, a factor that binds immunoglobulin Fcγ and cross-links VH3 clan B cell receptors (IgM), is the staphylococcal determinant for host immune suppression. To test this, vertebrate IgM was examined for protein A cross-linking. High VH3 binding activity occurred with human and guinea immunoglobulin, whereas mouse and rabbit immunoglobulins displayed little and no binding, respectively. Establishing a guinea pig model of S. aureus bloodstream infection, we show that protein A functions as a virulence determinant and suppresses host B cell responses. Immunization with SpAKKAA, which cannot bind immunoglobulin, elicits neutralizing antibodies that enable guinea pigs to develop protective immunity.
- Authors:
-
- Univ. of Chicago, IL (United States). Dept. of Microbiology; Argonne National Lab. (ANL), Argonne, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1201740
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- mBio (Online)
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2150-7511
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Kim, Hwan Keun, Falugi, Fabiana, Thomer, Lena, Missiakas, Dominique M., and Schneewind, Olaf. Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs. United States: N. p., 2015.
Web. doi:10.1128/mBio.02369-14.
Kim, Hwan Keun, Falugi, Fabiana, Thomer, Lena, Missiakas, Dominique M., & Schneewind, Olaf. Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs. United States. https://doi.org/10.1128/mBio.02369-14
Kim, Hwan Keun, Falugi, Fabiana, Thomer, Lena, Missiakas, Dominique M., and Schneewind, Olaf. 2015.
"Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs". United States. https://doi.org/10.1128/mBio.02369-14. https://www.osti.gov/servlets/purl/1201740.
@article{osti_1201740,
title = {Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs},
author = {Kim, Hwan Keun and Falugi, Fabiana and Thomer, Lena and Missiakas, Dominique M. and Schneewind, Olaf},
abstractNote = {Staphylococcus aureus infection is not associated with the development of protective immunity, and disease relapses occur frequently. We hypothesize that protein A, a factor that binds immunoglobulin Fcγ and cross-links VH3 clan B cell receptors (IgM), is the staphylococcal determinant for host immune suppression. To test this, vertebrate IgM was examined for protein A cross-linking. High VH3 binding activity occurred with human and guinea immunoglobulin, whereas mouse and rabbit immunoglobulins displayed little and no binding, respectively. Establishing a guinea pig model of S. aureus bloodstream infection, we show that protein A functions as a virulence determinant and suppresses host B cell responses. Immunization with SpAKKAA, which cannot bind immunoglobulin, elicits neutralizing antibodies that enable guinea pigs to develop protective immunity.},
doi = {10.1128/mBio.02369-14},
url = {https://www.osti.gov/biblio/1201740},
journal = {mBio (Online)},
issn = {2150-7511},
number = 1,
volume = 6,
place = {United States},
year = {Tue Jan 06 00:00:00 EST 2015},
month = {Tue Jan 06 00:00:00 EST 2015}
}
Web of Science
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