The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation

Xiong, Yi; Coradetti, Samuel T.; Li, Xin; Gritsenko, Marina A.; Clauss, Therese; Petyuk, Vlad; Camp, David; Smith, Richard; Cate, Jamie H. D.; Yang, Feng; Glass, N. Louise

doi:10.1016/j.fgb.2014.05.005

Title: The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation

Journal Article · Thu May 29 00:00:00 EDT 2014 · Fungal Genetics and Biology

DOI:https://doi.org/10.1016/j.fgb.2014.05.005· OSTI ID:1188945

^[1]; Coradetti, Samuel T. ^[1]; Li, Xin ^[2]; Gritsenko, Marina A. ^[3]; Clauss, Therese ^[3]; Petyuk, Vlad ^[3]; Camp, David ^[3]; Smith, Richard ^[3]; Cate, Jamie H. D. ^[4]; Yang, Feng ^[3]; Glass, N. Louise ^[1]

Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
Univ. of California, Berkeley, CA (United States). California Inst. for Quantitative Biosciences
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry

Improving cellulolytic enzyme production by plant biomass degrading fungi holds great potential in reducing costs associated with production of next-generation biofuels generated from lignocellulose. How fungi sense cellulosic materials and respond by secreting enzymes has mainly been examined by assessing function of transcriptional regulators and via transcriptional profiling. Here, we obtained global proteomic and phosphoproteomic profiles of the plant biomass degrading filamentous fungus Neurospora crassa grown on different carbon sources, i.e. sucrose, no carbon, and cellulose, by performing isobaric tags for relative and absolute quantification (iTRAQ)-based LC-MS/MS analyses. A comparison between proteomes and transcriptomes under identical carbon conditions suggests that extensive post-transcriptional regulation occurs in N. crassa in response to exposure to cellulosic material. Several hundred amino acid residues with differential phosphorylation levels on crystalline cellulose (Avicel) or carbon-free medium vs sucrose medium were identified, including phosphorylation sites in a major transcriptional activator for cellulase genes, CLR1, as well as a cellobionic acid transporter, CBT1. Mutation of phosphorylation sites on CLR1 did not have a major effect on transactivation of cellulase production, while mutation of phosphorylation sites in CBT1 increased its transporting capacity. Our data provides rich information at both the protein and phosphorylation levels of the early cellular responses to carbon starvation and cellulosic induction and aids in a greater understanding of the underlying post-transcriptional regulatory mechanisms in filamentous fungi.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; P41GM103493; AC02-05CH11231

OSTI ID:: 1188945

Alternate ID(s):: OSTI ID: 1222386; OSTI ID: 1407259

Report Number(s):: PNNL-SA-103196; 48135; 400412000

Journal Information:: Fungal Genetics and Biology, Vol. 72, Issue C; ISSN 1087-1845

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 54 works

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09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
Neurospora crassa
Proteome
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Cellulase
Carbon starvation
Plant biomass
Environmental Molecular Sciences Laboratory

Title: The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation

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