Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size

Bocharova, Vera; Sharp, Danna; Jones, Aaron; Cheng, Shiwang; Griffin, Philip J.; Agapov, Alexander L.; Voylov, Dmitry; Wang, Yangyang; Kisliuk, Alexander; Melman, Artem; Sokolov, Alexei P.

doi:10.1021/acs.chemmater.5b00187

Title: Enzyme Induced Formation of Monodisperse Hydrogel Nanoparticles Tunable in Size

Journal Article · Mon Mar 09 00:00:00 EDT 2015 · Chemistry of Materials

DOI:https://doi.org/10.1021/acs.chemmater.5b00187· OSTI ID:1185968

Bocharova, Vera ^[1]; Sharp, Danna ^[2]; Jones, Aaron ^[2]; Cheng, Shiwang ^[1]; Griffin, Philip J. ^[3]; Agapov, Alexander L. ^[2]; Voylov, Dmitry ^[2]; Wang, Yangyang ^[1]; Kisliuk, Alexander ^[1]; Melman, Artem ^[4]; Sokolov, Alexei P. ^[5]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Pennsylvania, Philadelphia, PA (United States)
Clarkson Univ., Potsdam, NY (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Here, we report a novel approach to synthesize monodisperse hydrogel nanoparticles that are tunable in size. The distinctive feature of our approach is the use of a multicopper oxidase enzyme, laccase, as both a biocatalyst and template for nanoparticle growth. We utilize the ferroxidase activity of laccase to initiate localized production of iron(III) cations from the oxidation of iron(II) cations. We demonstrate that nanoparticles are formed in a dilute polymer solution of alginate as a result of cross-linking between alginate and enzymatically produced iron(III) cations. Exerting control over the enzymatic reaction allows for nanometer-scale tuning of the hydrogel nanoparticle radii in the range of 30–100 nm. Moreover, the nanoparticles and their growth kinetics were characterized via dynamic light scattering, atomic force microscopy, and UV–vis spectroscopy. Our finding opens up a new avenue for the synthesis of tunable nanoscale hydrogel particles for biomedical applications.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-00OR22725; DMR-1408811

OSTI ID:: 1185968

Journal Information:: Chemistry of Materials, Vol. 27, Issue 7; ISSN 0897-4756

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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