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Title: Plasmids and packaging cell lines for use in phage display

Abstract

The invention relates to a novel phagemid display system for packaging phagemid DNA into phagemid particles which completely avoids the use of helper phage. The system of the invention incorporates the use of bacterial packaging cell lines which have been transformed with helper plasmids containing all required phage proteins but not the packaging signals. The absence of packaging signals in these helper plasmids prevents their DNA from being packaged in the bacterial cell, which provides a number of significant advantages over the use of both standard and modified helper phage. Packaged phagemids expressing a protein or peptide of interest, in fusion with a phage coat protein such as g3p, are generated simply by transfecting phagemid into the packaging cell line.

Inventors:
Publication Date:
Research Org.:
Los Alamos National Security, LLC, Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176463
Patent Number(s):
8,227,242
Application Number:
11/636,023
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Bradbury, Andrew M. Plasmids and packaging cell lines for use in phage display. United States: N. p., 2012. Web.
Bradbury, Andrew M. Plasmids and packaging cell lines for use in phage display. United States.
Bradbury, Andrew M. 2012. "Plasmids and packaging cell lines for use in phage display". United States. https://www.osti.gov/servlets/purl/1176463.
@article{osti_1176463,
title = {Plasmids and packaging cell lines for use in phage display},
author = {Bradbury, Andrew M.},
abstractNote = {The invention relates to a novel phagemid display system for packaging phagemid DNA into phagemid particles which completely avoids the use of helper phage. The system of the invention incorporates the use of bacterial packaging cell lines which have been transformed with helper plasmids containing all required phage proteins but not the packaging signals. The absence of packaging signals in these helper plasmids prevents their DNA from being packaged in the bacterial cell, which provides a number of significant advantages over the use of both standard and modified helper phage. Packaged phagemids expressing a protein or peptide of interest, in fusion with a phage coat protein such as g3p, are generated simply by transfecting phagemid into the packaging cell line.},
doi = {},
url = {https://www.osti.gov/biblio/1176463}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 24 00:00:00 EDT 2012},
month = {Tue Jul 24 00:00:00 EDT 2012}
}

Works referenced in this record:

A direct selection strategy for shotgun cloning and sequencing in the bacteriophage M13
journal, January 1994


Efficient Construction of a Large Collection of Phage-Displayed Combinatorial Peptide Libraries
journal, September 2005


Role of coronary angiography in acute coronary artery syndromes
journal, October 2002


A helper phage to improve single-chain antibody presentation in phage display
journal, January 2001


Engineering M13 for phage display
journal, September 2001


Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
journal, January 1985


Exploiting recombination in single bacteria to make large phage antibody libraries
journal, January 2000


Eliminating helper phage from phage display
journal, November 2006