skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: .beta.-glucosidase 5 (BGL5) compositions

Patent ·
OSTI ID:1176321

The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl5, and the corresponding BGL5 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL5, recombinant BGL5 proteins and methods for producing the same.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99GO10337
Assignee:
Danisco US Inc. (Palo Alto, CA)
Patent Number(s):
7,727,754
Application Number:
11/900,519
OSTI ID:
1176321
Resource Relation:
Patent File Date: 2007 Sep 12
Country of Publication:
United States
Language:
English

References (74)

Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography journal May 1998
Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis journal January 1988
A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast journal July 1994
Enzymatic Properties and Intracellular Localization of the Novel Trichoderma reesei  -Glucosidase BGLII (Cel1A) journal September 2002
Characteristics of fungal cellulases journal January 1991
Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites journal January 1985
Kinetics of cellulose saccharification by Trichoderma reesei cellulases journal January 2001
Reshaping human antibodies: grafting an antilysozyme activity journal March 1988
Separation of endo- and exo-type cellulases using a new affinity chromatography method journal April 1984
Characterization of cellulases by HPLC separation journal January 1983
CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora journal May 1990
 -Glucosidase and cellulase formation by a Trichoderma reesei mutant defective in constitutive  -glucosidase formation journal July 1990
Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase) journal January 1990
Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae journal April 1995
Developments in Directed Evolution for Improving Enzyme Functions journal August 2007
Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea journal January 1996
Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II journal January 1987
ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei journal April 2001
Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. journal November 1991
Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei journal September 2000
Purification and properties of an extracellular β-glucosidase from Lenzites trabea journal January 1978
Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae journal September 1987
Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning journal November 1999
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs journal September 1997
Properties of cellulolytic enzyme systems journal April 1985
New families in the classification of glycosyl hydrolases based on amino acid sequence similarities journal August 1993
Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans journal October 1981
Molecular Characterization and Heterologous Expression of the Gene Encoding a Low-Molecular-Mass Endoglucanase from Trichoderma reesei QM9414 journal January 1998
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA journal January 1982
Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs journal September 1995
The cellulase of Trichoderma viride. Purification, characterization and comparison of all detectable endoglucanases, exoglucanases and beta-glucosidases journal January 1985
Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene journal January 1986
Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain journal February 2001
Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli journal September 1994
Production of ethanol from biomasses. Part V Preparation and application of Trichoderma reesei mutants with enhanced .BETA.-glucosidase. journal January 1986
A radiation hybrid map of 18 growth factor, growth factor receptor, hormone receptor, or neurotransmitter receptor genes on the distal region of the long arm of chromosome 5 journal July 1992
Importance of Hydrogen-Bond Formation in Stabilizing the Transition State of Subtilisin
  • Wells, J. A.; Cunningham, B. C.; Graycar, T. P.
  • Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 317, Issue 1540, p. 415-423 https://doi.org/10.1098/rsta.1986.0051
journal April 1986
Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus journal September 1996
Reshaping human antibodies for therapy journal March 1988
Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27 journal October 1983
Improved oligonudeotide site-directed rautagenesis using M13 vectors journal January 1985
[79] Purification of all glycolytic enzymes from one muscle extract journal January 1982
[57] Rethinking your purification procedure book January 1990
Continuous cultures of fused cells secreting antibody of predefined specificity journal August 1975
cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast journal October 1997
Chemical chaperones increase the cellular activity of N370S β-glucosidase: A therapeutic strategy for Gaucher disease journal November 2002
Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast journal April 2001
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites journal January 1997
Genetically engineered peptide fusions for improved protein partitioning in aqueous two-phase systems journal March 2001
Basic local alignment search tool journal October 1990
A beta 3 integrin mutation abolishes ligand binding and alters divalent cation-dependent conformation journal August 1990
EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme journal March 1988
Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12 journal March 1998
A monoclonal antibody against the alkaline extracellular β-glucosidase from Trichoderma reesei: reactivity with other Trichoderma β-glucosidases journal September 1989
A novel genetic system to detect protein–protein interactions journal July 1989
Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae journal January 1987
Methods for measuring cellulase activities journal January 1988
Isolation and Characterization of a Mutant of Trichoderma reesei Showing Reduced Levels of Extracellular  -Glucosidase journal December 1989
Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae journal March 1988
Cellulase families and their genes journal September 1987
β-Glucosidase from the Grape Native Yeast Debaryomyces vanrijiae : Purification, Characterization, and Its Effect on Monoterpene Content of a Muscat Grape Juice journal February 2003
Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. journal November 1996
Replacing the complementarity-determining regions in a human antibody with those from a mouse journal May 1986
Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp journal June 2000
Cloning and Amplification of the Gene Encoding an Extracellular β-Glucosidase from Trichoderma reesei: Evidence for Improved Rates of Saccharification of Cellulosic Substrates journal June 1991
Viruses and gene silencing in plants book January 1999
Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography journal January 1987
Molecular Cloning and Expression of the Novel Fungal β-Glucosidase Genes from Humicola grisea and Trichoderma reesei journal April 1999
Cellulases of Trichoderma reesei journal January 1988
A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I journal March 1991
Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae. journal January 1996
Structure of Cellulases and Their Applications journal April 1997
Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae journal February 1996
Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction journal December 1999