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Title: Photoswitchable method for the ordered attachment of proteins to surfaces

Patent ·
OSTI ID:1176244
; ;

Described herein is a method for the attachment of proteins to any solid support with control over the orientation of the attachment. The method is extremely efficient, not requiring the previous purification of the protein to be attached, and can be activated by UV-light. Spatially addressable arrays of multiple protein components can be generated by using standard photolithographic techniques.

Research Organization:
Lawrence Livermore National Security, LLC, Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
7,700,334
Application Number:
11/688,171
OSTI ID:
1176244
Country of Publication:
United States
Language:
English

References (14)

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Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing journal May 2005
From combinatorial chemistry to chemical microarray journal June 2002
Local Photorelease of Caged Thymosin β4 in Locomoting Keratocytes Causes Cell Turning journal May 2001
Protein patterning journal April 1998
Protein trans -Splicing and Cyclization by a Naturally Split Intein from the dnaE Gene of Synechocystis Species PCC6803 journal March 2000
Editorial [Hot Topic: Polypeptide Chemical Ligation Tools in Protein Engineering (Guest Editor: Julio A. Camarero)] journal November 2005
Synthesis of Proteins by Native Chemical Ligation Using Fmoc-Based Chemistry journal November 2005
Chemoselective Attachment of Biologically Active Proteins to Surfaces by Expressed Protein Ligation and Its Application for “Protein Chip” Fabrication
  • Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.
  • Journal of the American Chemical Society, Vol. 126, Issue 45, p. 14730-14731 https://doi.org/10.1021/ja0456611
journal November 2004
Protein purification via temperature-dependent, intein-mediated cleavage from an immobilized metal affinity resin journal September 2006
Protein trans -splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803 journal August 1998
Membrane Protein Microarrays journal February 2002
New Developments for the Site-Specific Attachment of Protein to Surfaces journal January 2006
Selective Immobilization of Proteins onto Solid Supports through Split-Intein-Mediated Protein Trans-Splicing journal March 2006

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