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Title: Method to amplify variable sequences without imposing primer sequences

Patent ·
OSTI ID:1175987

The present invention provides methods of amplifying target sequences without including regions flanking the target sequence in the amplified product or imposing amplification primer sequences on the amplified product. Also provided are methods of preparing a library from such amplified target sequences.

Research Organization:
Univ. of California, Oakland, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-98ER62647
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
7,135,310
Application Number:
10/167,634
OSTI ID:
1175987
Resource Relation:
Patent File Date: 2002 Jun 11
Country of Publication:
United States
Language:
English

References (2)

Class-IIS restriction enzymes — a review journal April 1991
Structure and function of type II restriction endonucleases journal September 2001