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Title: Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis

Patent ·
OSTI ID:1175854

The present invention provides soluble cytochrome p450 reductase (CPR) proteins from Candida sp. having an altered N-terminal region which results in reduced hydrophobicity of the N-terminal region. Also provided are host cells comprising the subject soluble CPR proteins. In addition, the present invention provides nucleotide and corresponding amino acid sequences for soluble CPR proteins and vectors comprising the nucleotide sequences. Methods for producing a soluble CPR, for increasing production of a dicarboxylic acid, and for detecting a cytochrome P450 are also provided.

Research Organization:
Cognis Corporation, Ambler, PA (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-31-109-ENG-38
Assignee:
Cognis Corporation (Ambler, PA)
Patent Number(s):
7,083,960
Application Number:
10/272,017
OSTI ID:
1175854
Resource Relation:
Patent File Date: 2002 Oct 15
Country of Publication:
United States
Language:
English

References (8)

The N-Terminal Membrane Domain of Yeast NADPH-Cytochrome P450 (CYP) Oxidoreductase Is Not Required for Catalytic Activity in Sterol Biosynthesis or in Reconstitution of CYP Activity journal February 1998
Generation of a Complete, Soluble, and Catalytically Active Sterol 14α-Demethylase−Reductase Complex † journal July 1999
Genetically Engineered Modification of P450 Monooxygenases: Functional Analysis of the Amino-Terminal Hydrophobic Region and Hinge Region of the P450/Reductase Fused Enzyme journal December 1988
Truncations at the NH 2 Terminus of Rhodanese Destabilize the Enzyme and Decrease Its Heterologous Expression journal October 1998
Effects of Sequential Deletions of Residues from the N- or C-Terminus on the Functions of ε Subunit of the Chloroplast ATP Synthase † journal September 2001
A 36-Residue Peptide Contains All of the Information Required for 7B2-mediated Activation of Prohormone Convertase 2 journal July 1999
Dissection of NADPH-cytochrome P450 oxidoreductase into distinct functional domains. journal August 1994
Activities and Kinetic Mechanisms of Native and Soluble NADPH–Cytochrome P450 Reductase journal August 2001

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