Ultrahigh resolution multicolor colocalization of single fluorescent probes
Patent
·
OSTI ID:1175204
A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A novel multicolor sample-scanning confocal microscope was constructed which allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps using a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread-function of the microscope.
- Research Organization:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC03-76F00098
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Number(s):
- 6,844,150
- Application Number:
- 09/925,100
- OSTI ID:
- 1175204
- Country of Publication:
- United States
- Language:
- English
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