Label-free functional nucleic acid sensors for detecting target agents
A general methodology to design label-free fluorescent functional nucleic acid sensors using a vacant site approach and an abasic site approach is described. In one example, a method for designing label-free fluorescent functional nucleic acid sensors (e.g., those that include a DNAzyme, aptamer or aptazyme) that have a tunable dynamic range through the introduction of an abasic site (e.g., dSpacer) or a vacant site into the functional nucleic acids. Also provided is a general method for designing label-free fluorescent aptamer sensors based on the regulation of malachite green (MG) fluorescence. A general method for designing label-free fluorescent catalytic and molecular beacons (CAMBs) is also provided. The methods demonstrated here can be used to design many other label-free fluorescent sensors to detect a wide range of analytes. Sensors and methods of using the disclosed sensors are also provided.
- Research Organization:
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-08ER64568
- Assignee:
- The Board of Trustees of the University of Illinois (Urbana, IL)
- Patent Number(s):
- 8,933,210
- Application Number:
- 13/267,414
- OSTI ID:
- 1167209
- Resource Relation:
- Patent File Date: 2011 Oct 06
- Country of Publication:
- United States
- Language:
- English
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