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Title: NEAR-IR TWO PHOTON MICROSCOPY IMAGING OF SILICA NANOPARTICLES FUNCTIONALIZED WITH ISOLATED SENSITIZED Yb(III) CENTERS

Abstract

Bright nano objects emitting in the near infrared with a maximal cross section of 41.4 x 103 GM (Goppert Mayer), were prepared by implanting ca. 180 4,4 diethylaminostyryl 2,2 bipyridine (DEAS) Yb(III) complexes on the surface of 12 nm silica nanoparticles. The surface complexes ([DEAS Ln SiO2], Ln =Y,Yb) were characterized using IR, solid state NMR, UV Vis, EXAFS spectroscopies in combination with the preparation and characterization of similar molecular analogues by analytical techniques (IR, solution NMR, UV Vis, X ray crystallography) as well as DFT calculations. Starting from the partial dehydroxylation of the silica at 700 C on high vacuum having 0.8 OH.nm 2, the grafting of Ln(N(SiMe3)2)3 generate ≤SiO Ln(N(SiMe3)2)2, which upon thermal step and coordination of the DEAS chromophore yields (≤SiO)3Ln(DEAS). Surface and molecular analogues display similar properties, in terms of DEAS binding constants absorption maxima and luminescence properties (intense emission band assigned to a ligand centered CT fluorescence and life time) in the solid state, consistent with the molecular nature of the surface species. The densely functionalized nanoparticles can be dispersed via ultra-sonication in small ca. 15-20 nm aggregates (1 to 6 elementary particles) that were detected using two photon microscopy imaging at 720 nm excitation,more » making them promising nano objects for bio imaging.« less

Authors:
; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1166993
Report Number(s):
LBNL-6785E
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Chemistry of Materials
Additional Journal Information:
Journal Name: Chemistry of Materials
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; two photon, imaging, near-IR, ytterbium

Citation Formats

Lapadula, Giuseppe, Bourdolle, Adrien, Allouche, Florian, Conley, Matthew P., Maron, Laurent, Lukens, Wayne W., Guyot, Yannick, Andraud, Chantal, Brasselet, Sophie, Copé, ret, Christophe, Maury, Olivier, and Andersen, Richard A. NEAR-IR TWO PHOTON MICROSCOPY IMAGING OF SILICA NANOPARTICLES FUNCTIONALIZED WITH ISOLATED SENSITIZED Yb(III) CENTERS. United States: N. p., 2013. Web.
Lapadula, Giuseppe, Bourdolle, Adrien, Allouche, Florian, Conley, Matthew P., Maron, Laurent, Lukens, Wayne W., Guyot, Yannick, Andraud, Chantal, Brasselet, Sophie, Copé, ret, Christophe, Maury, Olivier, & Andersen, Richard A. NEAR-IR TWO PHOTON MICROSCOPY IMAGING OF SILICA NANOPARTICLES FUNCTIONALIZED WITH ISOLATED SENSITIZED Yb(III) CENTERS. United States.
Lapadula, Giuseppe, Bourdolle, Adrien, Allouche, Florian, Conley, Matthew P., Maron, Laurent, Lukens, Wayne W., Guyot, Yannick, Andraud, Chantal, Brasselet, Sophie, Copé, ret, Christophe, Maury, Olivier, and Andersen, Richard A. 2013. "NEAR-IR TWO PHOTON MICROSCOPY IMAGING OF SILICA NANOPARTICLES FUNCTIONALIZED WITH ISOLATED SENSITIZED Yb(III) CENTERS". United States. https://www.osti.gov/servlets/purl/1166993.
@article{osti_1166993,
title = {NEAR-IR TWO PHOTON MICROSCOPY IMAGING OF SILICA NANOPARTICLES FUNCTIONALIZED WITH ISOLATED SENSITIZED Yb(III) CENTERS},
author = {Lapadula, Giuseppe and Bourdolle, Adrien and Allouche, Florian and Conley, Matthew P. and Maron, Laurent and Lukens, Wayne W. and Guyot, Yannick and Andraud, Chantal and Brasselet, Sophie and Copé and ret, Christophe and Maury, Olivier and Andersen, Richard A.},
abstractNote = {Bright nano objects emitting in the near infrared with a maximal cross section of 41.4 x 103 GM (Goppert Mayer), were prepared by implanting ca. 180 4,4 diethylaminostyryl 2,2 bipyridine (DEAS) Yb(III) complexes on the surface of 12 nm silica nanoparticles. The surface complexes ([DEAS Ln SiO2], Ln =Y,Yb) were characterized using IR, solid state NMR, UV Vis, EXAFS spectroscopies in combination with the preparation and characterization of similar molecular analogues by analytical techniques (IR, solution NMR, UV Vis, X ray crystallography) as well as DFT calculations. Starting from the partial dehydroxylation of the silica at 700 C on high vacuum having 0.8 OH.nm 2, the grafting of Ln(N(SiMe3)2)3 generate ≤SiO Ln(N(SiMe3)2)2, which upon thermal step and coordination of the DEAS chromophore yields (≤SiO)3Ln(DEAS). Surface and molecular analogues display similar properties, in terms of DEAS binding constants absorption maxima and luminescence properties (intense emission band assigned to a ligand centered CT fluorescence and life time) in the solid state, consistent with the molecular nature of the surface species. The densely functionalized nanoparticles can be dispersed via ultra-sonication in small ca. 15-20 nm aggregates (1 to 6 elementary particles) that were detected using two photon microscopy imaging at 720 nm excitation, making them promising nano objects for bio imaging.},
doi = {},
url = {https://www.osti.gov/biblio/1166993}, journal = {Chemistry of Materials},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 12 00:00:00 EST 2013},
month = {Sat Jan 12 00:00:00 EST 2013}
}