Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell
Abstract
We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 °C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.
- Authors:
-
- Stony Brook Univ., NY (United States) Dept. of Physics and Astronomy
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Stony Brook Univ., NY (United States) Dept. of Biochemistry and Cell Biology
- Publication Date:
- Research Org.:
- Stony Brook Univ., NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1164142
- Grant/Contract Number:
- FG02-04ER46128
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Physical Review Letters
- Additional Journal Information:
- Journal Volume: 103; Journal Issue: 19; Journal ID: ISSN 0031-9007
- Publisher:
- American Physical Society (APS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Huang, Xiaojing, Nelson, Johanna, Kirz, Janos, Lima, Enju, Marchesini, Stefano, Miao, Huijie, Neiman, Aaron M., Shapiro, David, Steinbrener, Jan, Stewart, Andrew, Turner, Joshua J., and Jacobsen, Chris. Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell. United States: N. p., 2009.
Web. doi:10.1103/PhysRevLett.103.198101.
Huang, Xiaojing, Nelson, Johanna, Kirz, Janos, Lima, Enju, Marchesini, Stefano, Miao, Huijie, Neiman, Aaron M., Shapiro, David, Steinbrener, Jan, Stewart, Andrew, Turner, Joshua J., & Jacobsen, Chris. Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell. United States. https://doi.org/10.1103/PhysRevLett.103.198101
Huang, Xiaojing, Nelson, Johanna, Kirz, Janos, Lima, Enju, Marchesini, Stefano, Miao, Huijie, Neiman, Aaron M., Shapiro, David, Steinbrener, Jan, Stewart, Andrew, Turner, Joshua J., and Jacobsen, Chris. 2009.
"Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell". United States. https://doi.org/10.1103/PhysRevLett.103.198101. https://www.osti.gov/servlets/purl/1164142.
@article{osti_1164142,
title = {Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell},
author = {Huang, Xiaojing and Nelson, Johanna and Kirz, Janos and Lima, Enju and Marchesini, Stefano and Miao, Huijie and Neiman, Aaron M. and Shapiro, David and Steinbrener, Jan and Stewart, Andrew and Turner, Joshua J. and Jacobsen, Chris},
abstractNote = {We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 °C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.},
doi = {10.1103/PhysRevLett.103.198101},
url = {https://www.osti.gov/biblio/1164142},
journal = {Physical Review Letters},
issn = {0031-9007},
number = 19,
volume = 103,
place = {United States},
year = {Sun Nov 01 00:00:00 EDT 2009},
month = {Sun Nov 01 00:00:00 EDT 2009}
}
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