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Title: Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates

Abstract

The lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. Likewise, an understanding of the toxic effects of acetate on the fermentation microorganism and the efficient utilization of mixed sugars of glucose and xylose in the presence of hydrolysate inhibitors is crucial for economic biofuel production.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
OSTI Identifier:
1160173
Report Number(s):
NREL/JA-5100-61892
Journal ID: ISSN 1754-6834; MainId:24294;UUID:f4a2d632-c3cb-e311-8d7c-d89d67132a6d;MainAdminID:12770
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 7; Journal Issue: 9, September 2014; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Zymomonas mobilis; acetate; xylose; transcriptomics; microarray; toxicity; pretreatment

Citation Formats

Yang, Shihui, Franden, M. A., Brown, S. D., Chou, Y. C., Pienkos, P. T., and Zhang, Min. Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates. United States: N. p., 2014. Web. doi:10.1186/s13068-014-0140-8.
Yang, Shihui, Franden, M. A., Brown, S. D., Chou, Y. C., Pienkos, P. T., & Zhang, Min. Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates. United States. https://doi.org/10.1186/s13068-014-0140-8
Yang, Shihui, Franden, M. A., Brown, S. D., Chou, Y. C., Pienkos, P. T., and Zhang, Min. 2014. "Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates". United States. https://doi.org/10.1186/s13068-014-0140-8. https://www.osti.gov/servlets/purl/1160173.
@article{osti_1160173,
title = {Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates},
author = {Yang, Shihui and Franden, M. A. and Brown, S. D. and Chou, Y. C. and Pienkos, P. T. and Zhang, Min},
abstractNote = {The lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. Likewise, an understanding of the toxic effects of acetate on the fermentation microorganism and the efficient utilization of mixed sugars of glucose and xylose in the presence of hydrolysate inhibitors is crucial for economic biofuel production.},
doi = {10.1186/s13068-014-0140-8},
url = {https://www.osti.gov/biblio/1160173}, journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 9, September 2014,
volume = 7,
place = {United States},
year = {Tue Sep 30 00:00:00 EDT 2014},
month = {Tue Sep 30 00:00:00 EDT 2014}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 40 works
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Works referenced in this record:

Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates
journal, January 2013


Effect of organic acids on the growth and fermentation of ethanologenicEscherichia coli LY01
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Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli
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Bacteria engineered for fuel ethanol production: current status
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Zymomonas mobilis: an alternative ethanol producer
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Complete Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. mobilis Centrotype ATCC 29191
journal, October 2012


Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. pomaceae Lectotype Strain ATCC 29192
journal, July 2011


Complete Genome Sequence of the Ethanol Producer Zymomonas mobilis NCIMB 11163
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The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4
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Systems Biology Analysis of Zymomonas mobilis ZM4 Ethanol Stress Responses
journal, July 2013


Genome-scale modeling and in silico analysis of ethanologenic bacteria Zymomonas mobilis
journal, November 2010


Biotechnological potential of respiring Zymomonas mobilis: A stoichiometric analysis of its central metabolism
journal, May 2013


Kinetic modeling to optimize pentose fermentation inZymomonas mobilis
journal, January 2006


DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4
journal, October 2010


Use of an EZ-Tn5-based random mutagenesis system to create a Zymomonas mobilis with significant tolerance to heat stress and malnutrition
journal, May 2013


Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates
journal, January 2013


Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
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Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering.
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Over-expression of xylulokinase in a xylose-metabolising recombinant strain of Zymomonas mobilis
journal, March 2005


Cofermentation of Glucose, Xylose,and Arabinose by Genomic DNA-IntegratedXylose/Arabinose Fermenting Strainof <E1>Zymomonas mobilis</E1> AX101
journal, January 2002


Cofermentation of glucose, xylose, and arabinose by mixed cultures of two genetically engineeredZymomonas mobilis strains
journal, March 1998


Transcriptome profiling of Zymomonas mobilis under ethanol stress
journal, January 2012


Paradigm for industrial strain improvement identifies sodium acetate tolerance loci in Zymomonas mobilis and Saccharomyces cerevisiae
journal, May 2010


An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli
journal, January 2009


Improved genome annotation for Zymomonas mobilis
journal, October 2009


Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations
journal, January 2009


STRING 8--a global view on proteins and their functional interactions in 630 organisms
journal, January 2009


Transcriptome profiling of Zymomonas mobilis under furfural stress
journal, May 2012


Metabolomic and transcriptomic stress response of Escherichia coli
journal, January 2010


Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4
journal, July 2011


Reduction of xylose to xylitol catalyzed by glucose–fructose oxidoreductase from Zymomonas mobilis
journal, April 2009


Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress
journal, January 2012


Improving xylose utilization by recombinant Zymomonas mobilis strain 8b through adaptation using 2-deoxyglucose
journal, January 2014


Kinetic modeling to optimize pentose fermentation inZymomonas mobilis
journal, January 2006


Genome-scale modeling and in silico analysis of ethanologenic bacteria Zymomonas mobilis
journal, November 2010


Zymomonas mobilis: an alternative ethanol producer
journal, January 2006


Bacteria engineered for fuel ethanol production: current status
journal, December 2003


DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4
journal, October 2010


Transcriptome profiling of Zymomonas mobilis under furfural stress
journal, May 2012


Use of an EZ-Tn5-based random mutagenesis system to create a Zymomonas mobilis with significant tolerance to heat stress and malnutrition
journal, May 2013


Biotechnological potential of respiring Zymomonas mobilis: A stoichiometric analysis of its central metabolism
journal, May 2013


Ethanol cycle in an ethanologenic bacterium
journal, June 2002


Metabolomic and transcriptomic stress response of Escherichia coli
journal, January 2010


Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates
journal, January 2013


Improved genome annotation for Zymomonas mobilis
journal, October 2009


The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4
journal, December 2004


Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995


Identification and Inactivation of Genetic Loci Involved with Lactobacillus acidophilus Acid Tolerance
journal, September 2004


Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations
journal, January 2009


Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress
journal, January 2012


An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli
journal, January 2009


Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli
journal, January 2009


Transcriptome profiling of Zymomonas mobilis under ethanol stress
journal, January 2012


Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates
journal, January 2013


Improving xylose utilization by recombinant Zymomonas mobilis strain 8b through adaptation using 2-deoxyglucose
journal, January 2014


Systems Biology Analysis of Zymomonas mobilis ZM4 Ethanol Stress Responses
journal, July 2013


Elucidation of Zymomonas mobilis physiology and stress responses by quantitative proteomics and transcriptomics
journal, May 2014


Works referencing / citing this record:

Furfural-tolerant Zymomonas mobilis derived from error-prone PCR-based whole genome shuffling and their tolerant mechanism
journal, February 2018


Zymomonas mobilis as a model system for production of biofuels and biochemicals
journal, September 2016


Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate
journal, March 2015


Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars
journal, September 2016


Proteomic and metabolomic analysis of the cellular biomarkers related to inhibitors tolerance in Zymomonas mobilis ZM4
journal, October 2018


Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era
journal, March 2019


Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis
journal, February 2018


Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate
journal, March 2015


Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms
journal, April 2018