Metabolic profiling reveals altered sugar and secondary metabolism in response to UGPase overexpression in Populus

Payyavula, Raja S.; Tschaplinski, Timothy J.; Jawdy, Sara; Sykes, Robert; Tuskan, Gerald A.; Kalluri, Udaya C.

doi:10.1186/s12870-014-0265-8

Title: Metabolic profiling reveals altered sugar and secondary metabolism in response to UGPase overexpression in Populus

Journal Article · Tue Oct 07 00:00:00 EDT 2014 · BMC Plant Biology

DOI:https://doi.org/10.1186/s12870-014-0265-8· OSTI ID:1159413

Payyavula, Raja S. ^[1]; Tschaplinski, Timothy J. ^[1]; Jawdy, Sara ^[1]; Sykes, Robert ^[2]; Tuskan, Gerald A. ^[1]; Kalluri, Udaya C. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)

Background: UDP-glucose pyrophopharylase (UGPase) is a sugar metabolizing enzyme (E.C. 2.7.7.9) that catalyzes a reversible reaction of UDP-glucose and pyrophosphate from glucose-1-phosphate and uridine triphosphate glucose. UDP-glucose is a key intermediate sugar that is channeled to multiple metabolic pathways. The functional role of UGPase in woody plants such as Populus is poorly understood. Results: We characterized the functional role of UGPase in Populus deltoides by overexpressing a native gene. Overexpression of the native gene resulted in increased leaf area and leaf-to-shoot biomass ratio but decreased shoot and root growth. Metabolomic analyses showed that manipulation of UGPase results in perturbations in primary as well as secondary metabolism resulting in reduced sugar and starch levels and increased phenolics such as caffeoyl- and feruloyl conjugates. While cellulose and lignin levels in the cell walls were not significantly altered, the syringyl-to-guaiacyl ratio was significantly reduced. Conclusions: These results demonstrate that UGPase plays a key role in the tightly coupled primary and secondary metabolic pathways and perturbation in its function results in pronounced effects on growth and metabolism outside of cell wall biosynthesis of Populus.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC36-08GO28308

OSTI ID:: 1159413

Alternate ID(s):: OSTI ID: 1242474

Report Number(s):: NREL/JA-5100-63190; KP1601050; ERKP695

Journal Information:: BMC Plant Biology, Vol. 14, Issue 265; ISSN 1471-2229

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 42 works

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