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Title: Disposable and removable nucleic acid extraction and purification cartridges for automated flow-through systems

Abstract

Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument.

Inventors:
Publication Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1157562
Patent Number(s):
8,828,716
Application Number:
12/038,981
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Regan, John Frederick. Disposable and removable nucleic acid extraction and purification cartridges for automated flow-through systems. United States: N. p., 2014. Web.
Regan, John Frederick. Disposable and removable nucleic acid extraction and purification cartridges for automated flow-through systems. United States.
Regan, John Frederick. 2014. "Disposable and removable nucleic acid extraction and purification cartridges for automated flow-through systems". United States. https://www.osti.gov/servlets/purl/1157562.
@article{osti_1157562,
title = {Disposable and removable nucleic acid extraction and purification cartridges for automated flow-through systems},
author = {Regan, John Frederick},
abstractNote = {Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument.},
doi = {},
url = {https://www.osti.gov/biblio/1157562}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 09 00:00:00 EDT 2014},
month = {Tue Sep 09 00:00:00 EDT 2014}
}

Works referenced in this record:

Process for isolating nucleic acid
patent, August 1993


Apparatus and method for isolating a nucleic acid from a sample
patent, May 2007


Automated nucleic acid assay system
patent-application, February 2003


Microfluidic integrated microarrays for biological detection
patent-application, May 2005


Devices,systems and methods for multi-dimensional separation
patent-application, August 2006


Protein separation column
patent-application, November 2006


Cartridge for nucleic acid separation and purification and method for producing the same
patent-application, June 2007


Systems and Methods for Testing Using Microfluidic Chips
patent-application, November 2008


Fluid Concentrator, Autologous Concentrated Body Fluids, and Uses Thereof
patent-application, October 2010