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Title: Oxygen-resistant hydrogenases and methods for designing and making same

Patent ·
OSTI ID:1127083
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The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
8,663,958
Application Number:
12/360,756
OSTI ID:
1127083
Country of Publication:
United States
Language:
English

References (17)

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Measurement of gas production of algal clones patent March 1992
Methods and compositions for producing metabolic products for algae patent December 1993
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Phaffia rhodozyma mutants, process for producing .beta.-carotene and use of .beta.-carotene rich biomass patent November 1997
Process for selection of Oxygen-tolerant algal mutants that produce H.sub.2 patent February 1999
System for rapid biohydrogen phenotypic screening of microorganisms using a chemochromic sensor patent September 2002
Methods and compositions for evolving hydrogenase genes patent November 2006
Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms patent-application December 2001
Process and apparatus for isolating and continuosly cultivating, harvesting, and processing of a substantially pure form of a desired species of algae patent-application March 2002
Oxygen-resistant hydrogenases and methods for designing and making same patent-application October 2006
Methods and compositions for evolving hydrogenase genes patent-application January 2007
Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii : Fe-hydrogenase from journal February 2002
Amino Acid Replacements at the H 2 -Activating Site of the NAD-Reducing Hydrogenase from Alcaligenes eutrophus † journal October 1999
Classification and phylogeny of hydrogenases journal August 2001
Accumulation of O2-tolerant phenotypes in H2-producing strains of Chlamydomonas reinhardtii by sequential applications of chemical mutagenesis and selection journal November 2002

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