Methods and compounds for chemical ligation

Church, George M.; Sismour, A. Michael

Title: Methods and compounds for chemical ligation

Patent · Tue Jul 09 00:00:00 EDT 2013

OSTI ID:1087892

Church, George M.; Sismour, A. Michael

Compositions and methods for chemical ligation are provided. Methods for nucleic acid sequencing, nucleic acid assembly and nucleic acid synthesis are also provided.

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Research Organization:: Harvard College (Cambridge, MA)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-02ER63445

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: 8,481,258

Application Number:: 12/628,518

OSTI ID:: 1087892

Country of Publication:: United States

Language:: English

References (34)

RNA Hybridization to DNA Coupled with Cyanogen-Bromide-Activated Sephadex. The Purification of Polyoma Messenger RNA Siddell, Stuart G. European Journal of Biochemistry, Vol. 92, Issue 2 https://doi.org/10.1111/j.1432-1033.1978.tb12785.x	journal	December 1978
SNPs analysis by use of oligonucleotides containing 2′-O-methyl-2-thiouridines Okamoto, Itaru; Seio, Kohji; Sekine, Mitsuo Nucleic Acids Symposium Series, Vol. 49, Issue 1 https://doi.org/10.1093/nass/49.1.123	journal	September 2005
Phosphoramidate-phosphodiester oligonucleotide chimera as primers Ellis, Nicole M.; Kuimelis, Robert G.; Heiner, Cheryl https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6043070 US Patent Document 6,043,070	patent	March 2000
In vitro selection of optimal DNA substrates for ligation by a water-soluble carbodiimide Harada, Kazuo; Orgel, LeslieE. Journal of Molecular Evolution, Vol. 38, Issue 6 https://doi.org/10.1007/BF00175874	journal	June 1994
Unambiguous demonstration of triple-helix-directed gene modification Barre, F. -X.; Ait-Si-Ali, S.; Giovannangeli, C. Proceedings of the National Academy of Sciences, Vol. 97, Issue 7 https://doi.org/10.1073/pnas.97.7.3084	journal	March 2000
Non-radioactive nucleic acid hybridization probes Al-Hakim, Ali; Hull, Roger https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4996142 US Patent Document 4,996,142	patent	February 1991
DNA sequencing by parallel oligonucleotide extensions Macevicz, Stephen C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5750341 US Patent Document 5,750,341	patent	May 1998
Chemical ligation as a method for the assembly of double-stranded nucleic acids: Modifications and local structure studies Dolinnaya, N. G.; Shabarova, Z. A. Russian Chemical Bulletin, Vol. 45, Issue 8 https://doi.org/10.1007/BF01457755	journal	August 1996
The use of BrCN for assembling modified DNA duplexes and DNA-RNA hybrids; comparison with water-soluble carbodiimide Dolinnaya, Nina G.; Sokolova, Nadejda I.; Ashirbekova, Dana T. Nucleic Acids Research, Vol. 19, Issue 11 https://doi.org/10.1093/nar/19.11.3067	journal	January 1991
Advanced sequencing technologies: methods and goals Shendure, Jay; Mitra, Robi D.; Varma, Chris Nature Reviews Genetics, Vol. 5, Issue 5 https://doi.org/10.1038/nrg1325	journal	May 2004
Kinetic model studies on the chemical ligation of oligonucleotides via hydrazone formation Achilles, K.; Kiedrowski, G. v. Bioorganic & Medicinal Chemistry Letters, Vol. 15, Issue 4 https://doi.org/10.1016/j.bmcl.2004.11.068	journal	February 2005
New chemically reactive dsDNAs containing single internucleotide monophosphoryldithio links: reactivity of 5′-mercapto-oligodeoxyribonucleotides Metelev, Valeri G.; Borisova, Oxana A.; Volkov, Eugene M. Nucleic Acids Research, Vol. 29, Issue 19 https://doi.org/10.1093/nar/29.19.4062	journal	October 2001
Fluorescent in situ sequencing on polymerase colonies Mitra, Robi D.; Shendure, Jay; Olejnik, Jerzy Analytical Biochemistry, Vol. 320, Issue 1 https://doi.org/10.1016/S0003-2697(03)00291-4	journal	September 2003
Use of a new retrieving adaptor in the cloning of a synthetic human insulin A-chain gene Scarpulla, Richard C.; Narang, Saran; Wu, Ray Analytical Biochemistry, Vol. 121, Issue 2 https://doi.org/10.1016/0003-2697(82)90493-6	journal	April 1982
Template controlled coupling and recombination of oligonucleotide blocks containing thiophosphoryl groups Gryaznov, Sergei M.; Letsinger, Robert L. Nucleic Acids Research, Vol. 21, Issue 6 https://doi.org/10.1093/nar/21.6.1403	journal	January 1993
Template-Directed Assembly and Characterization of Metallosalen−DNA Conjugates Czlapinski, Jennifer L.; Sheppard, Terry L. Bioconjugate Chemistry, Vol. 16, Issue 1 https://doi.org/10.1021/bc049753r	journal	December 2004
Site-directed modification of DNA duplexes by chemical ligation Dolinnaya, N. G.; Sokolova, N. I.; Gryaznova, O. I. Nucleic Acids Research, Vol. 16, Issue 9 https://doi.org/10.1093/nar/16.9.3721	journal	January 1988
Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups Letsinger, Robert L.; Gryaznov, Sergei https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5476925 US Patent Document 5,476,925	patent	December 1995
Chemical reactions within DNA duplexes Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent Sokolova, N. I.; Ashirbekova, D. T.; Dolinnaya, N. G. FEBS Letters, Vol. 232, Issue 1 https://doi.org/10.1016/0014-5793(88)80406-X	journal	May 1988
Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays Brenner, Sydney; Johnson, Maria; Bridgham, John Nature Biotechnology, Vol. 18, Issue 6 https://doi.org/10.1038/76469	journal	June 2000
Oligonucleotide circularization by template-directed chemical ligation DOlinnaya, Nina G.; Blumenfeld, Marta; Merenkova, Irena N. Nucleic Acids Research, Vol. 21, Issue 23 https://doi.org/10.1093/nar/21.23.5403	journal	January 1993
DNA sequencing by parallel oligonucleotide extensions Macevicz, Stephen C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6306597 US Patent Document 6,306,597	patent	October 2001
Chemical ligation of oligodeoxyribonucleotides on circular DNA templates Selvasekaran, J.; Turnbull, K. D. Nucleic Acids Research, Vol. 27, Issue 2 https://doi.org/10.1093/nar/27.2.624	journal	January 1999
Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties Gryaznov, Sergei; Schultz, Ronald G.; Chen, Jer-Kang https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5599922 US Patent Document 5,599,922	patent	February 1997
Oligonucleotide mediated nucleic acid recombination Crameri, Andreas; Stemmer, Willem P. C.; Minshull, Jeremy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6376246 US Patent Document 6,376,246	patent	April 2002
DNA SEQUENCING:A Sequencing Method Based on Real-Time Pyrophosphate Ronaghi, M. Science, Vol. 281, Issue 5375 https://doi.org/10.1126/science.281.5375.363	journal	July 1998
Surprising fidelity of template-directed chemical ligation of oligonucleotides James, Kenneth D.; Ellington, Andrew D. Chemistry & Biology, Vol. 4, Issue 8 https://doi.org/10.1016/S1074-5521(97)90245-3	journal	August 1997
Oligonucleotide N3'-->P5' phosphoramidates as antisense agents Gryaznov, S. Nucleic Acids Research, Vol. 24, Issue 8 https://doi.org/10.1093/nar/24.8.1508	journal	April 1996
The use of diaminopurine to investigate structural properties of nucleic acids and molecular recognition between ligands and DNA Bailly, C. Nucleic Acids Research, Vol. 26, Issue 19 https://doi.org/10.1093/nar/26.19.4309	journal	October 1998
Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot Pall, G. S.; Codony-Servat, C.; Byrne, J. Nucleic Acids Research, Vol. 35, Issue 8 https://doi.org/10.1093/nar/gkm112	journal	March 2007
Template-directed synthesis: use of a reversible reaction Goodwin, Jay T.; Lynn, David G. Journal of the American Chemical Society, Vol. 114, Issue 23 https://doi.org/10.1021/ja00049a067	journal	November 1992
Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome Shendure, J. Science, Vol. 309, Issue 5741 https://doi.org/10.1126/science.1117389	journal	September 2005
Studies on polynucleotides. LXXXVII. The joining of short deoxyribopolynucleotides by DNA-joining enzymes. Gupta, N. K.; Ohtsuka, E.; Weber, H. Proceedings of the National Academy of Sciences, Vol. 60, Issue 1 https://doi.org/10.1073/pnas.60.1.285	journal	May 1968
Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene Shabarova, Zoe A.; Merenkova, Irena N.; Oretskaya, Tatyana S. Nucleic Acids Research, Vol. 19, Issue 15 https://doi.org/10.1093/nar/19.15.4247	journal	January 1991

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