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Title: Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater

Abstract

Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Mississippi State Univ., Starkville, MS (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1079530
Report Number(s):
GO8602552
Journal ID: ISSN 0268-2575
DOE Contract Number:  
FG36-06GO86025
Resource Type:
Journal Article
Journal Name:
Journal of Chemical Technology and Biotechnology (1986)
Additional Journal Information:
Journal Volume: 86; Journal Issue: 1; Journal ID: ISSN 0268-2575
Country of Publication:
United States
Language:
English
Subject:
Bio-fuel, Municipal wastewater, Oleaginous microorganisms, Primary effluent

Citation Formats

Hall, Jacqueline, Hetrick, Mary, French, Todd, Hernandez, Rafael, Donaldson, Janet, Mondala, Andro, and Holmes, William. Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater. United States: N. p., 2011. Web. doi:10.1002/jctb.2506.
Hall, Jacqueline, Hetrick, Mary, French, Todd, Hernandez, Rafael, Donaldson, Janet, Mondala, Andro, & Holmes, William. Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater. United States. https://doi.org/10.1002/jctb.2506
Hall, Jacqueline, Hetrick, Mary, French, Todd, Hernandez, Rafael, Donaldson, Janet, Mondala, Andro, and Holmes, William. 2011. "Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater". United States. https://doi.org/10.1002/jctb.2506. https://www.osti.gov/servlets/purl/1079530.
@article{osti_1079530,
title = {Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater},
author = {Hall, Jacqueline and Hetrick, Mary and French, Todd and Hernandez, Rafael and Donaldson, Janet and Mondala, Andro and Holmes, William},
abstractNote = {Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.},
doi = {10.1002/jctb.2506},
url = {https://www.osti.gov/biblio/1079530}, journal = {Journal of Chemical Technology and Biotechnology (1986)},
issn = {0268-2575},
number = 1,
volume = 86,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2011},
month = {Sat Jan 01 00:00:00 EST 2011}
}