Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
Abstract
Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.
- Inventors:
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1079492
- Patent Number(s):
- 8,283,150
- Application Number:
- 12/247,594
- Assignee:
- Alliance for Sustainable Energy, LLC (Golden, CO)
- DOE Contract Number:
- AC36-08GO28308
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Adney, William S., Baker, John O., Decker, Stephen R., Chou, Yat-Chen, Himmel, Michael E., and Ding, Shi-You. Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum. United States: N. p., 2012.
Web.
Adney, William S., Baker, John O., Decker, Stephen R., Chou, Yat-Chen, Himmel, Michael E., & Ding, Shi-You. Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum. United States.
Adney, William S., Baker, John O., Decker, Stephen R., Chou, Yat-Chen, Himmel, Michael E., and Ding, Shi-You. 2012.
"Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum". United States. https://www.osti.gov/servlets/purl/1079492.
@article{osti_1079492,
title = {Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum},
author = {Adney, William S. and Baker, John O. and Decker, Stephen R. and Chou, Yat-Chen and Himmel, Michael E. and Ding, Shi-You},
abstractNote = {Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.},
doi = {},
url = {https://www.osti.gov/biblio/1079492},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 09 00:00:00 EDT 2012},
month = {Tue Oct 09 00:00:00 EDT 2012}
}
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