skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Multiplex detection of respiratory pathogens

Patent ·
OSTI ID:1058899
 [1];  [2];  [3]
  1. Brentwood, CA
  2. Livermore, CA
  3. Albany, CA
+ Show Author Affiliations

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
8,232,058
Application Number:
12/161,291
OSTI ID:
1058899
Country of Publication:
United States
Language:
English

References (25)

A Sensitive, Specific, and Cost-Effective Multiplex Reverse Transcriptase-PCR Assay for the Detection of Seven Common Respiratory Viruses in Respiratory Samples journal May 2004
Rapid Detection and Identification of Human Adenovirus Species by Adenoplex, a Multiplex PCR-Enzyme Hybridization Assay journal September 2004
Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care journal September 2007
Genetic analysis of human H2N2 and early H3N2 influenza viruses, 1957–1972: evidence for genetic divergence and multiple reassortment events journal October 2004
Influenza A viruses in feral Canadian ducks: extensive reassortment in nature journal August 2004
A computer program for selection of oligonucleotide primers for polymerase chain reactions journal January 1990
Large-Scale Sequence Analysis of Avian Influenza Isolates journal March 2006
A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents journal April 2005
Comparison of Real-Time PCR Assays with Fluorescent-Antibody Assays for Diagnosis of Respiratory Virus Infections in Children journal July 2006
Evolutionary characterization of the six internal genes of H5N1 human influenza A virus journal May 2000
Research Profiles: Liquid array single-handedly detects bounty of BW agents journal May 2003
Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4 journal April 2004
Characterizations of the human parainfluenza type 2 virus gene encoding the L protein and the intergenic sequences journal May 1991
Stable and Noncompetitive RNA Internal Control for Routine Clinical Diagnostic Reverse Transcription-PCR journal March 2004
Design Strategies and Performance of Custom DNA Sequencing Primers journal September 1999
Evolution of H5 subtype avian influenza A viruses in North America journal October 1997
Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents journal April 2003
Antigenic and genetic diversity among swine influenza A H1N1 and H1N2 viruses in Europe journal April 2002
Clinical and Financial Benefits of Rapid Detection of Respiratory Viruses: an Outcomes Study journal January 2000
Molecular changes associated with the transmission of avian influenza a H5N1 and H9N2 viruses to humans journal November 2001
Direct Detection of Respiratory Syncytial Virus, Parainfluenza Virus, and Adenovirus in Clinical Respiratory Specimens by a Multiplex Reverse Transcription-PCR Assay journal January 1998
Comparative Analysis of Evolutionary Mechanisms of the Hemagglutinin and Three Internal Protein Genes of Influenza B Virus: Multiple Cocirculating Lineages and Frequent Reassortment of the NP, M, and NS Genes journal May 1999
Transmission of Eurasian avian H2 influenza virus to shorebirds in North America journal December 1999
Origin of the Pandemic 1957 H2 Influenza A Virus and the Persistence of Its Possible Progenitors in the Avian Reservoir journal June 1993
Comparison of the Directigen Flu A+B Test, the QuickVue Influenza Test, and Clinical Case Definition to Viral Culture and Reverse Transcription-PCR for Rapid Diagnosis of Influenza Virus Infection journal August 2003

Similar Records

A multiplexed reverse transcriptase PCR assay for identification of viral respiratory pathogens at point-of-care
Journal Article · Wed Apr 11 00:00:00 EDT 2007 · Journal of Clinical Microbiology · OSTI ID:1058899
+5 more
Multiplex detection of agricultural pathogens
Patent · Tue Sep 14 00:00:00 EDT 2010 · OSTI ID:1058899
Multiplex detection of agricultural pathogens
Patent · Tue Jan 15 00:00:00 EST 2013 · OSTI ID:1058899
+2 more

Related Subjects

60 APPLIED LIFE SCIENCES