Identification of the genomic locus for the human Rieske Fe-S Protein gene on Chromosome 19q12
Conference
·
OSTI ID:105140
- Sonoma State Univ. (United States)
We have identified the chromosomal location of the human Rieske Iron-Sulfur Protein (UQCRFS1) gene. Mapping by hybridization to a panel of monochromosomal hybrid cell lines indicated that the gene was either on chromosome 19 or 22. By screening a human chromosome 19 specific genomic cosmid library with an oligonucleotide probe made from the published Rieske cDNA sequence, we identified a corresponding cosmid. Portions of this cosmid were sequenced directly. The exon, exon:intron junction, and flanking sequences verified that this cosmid contains the genomic locus. Fluorescent in situ hybridization (FISH) was performed to localize this cosmid to chromosome band 19q12.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 105140
- Report Number(s):
- UCRL-ID-114972-2; CONF-9405328-Absts.; ON: DE95014118; TRN: 95:006489-0004
- Resource Relation:
- Conference: Science and Engineering Research Semester (SERS) student symposium, Livermore, CA (United States), 6 May 1994; Other Information: PBD: 6 May 1994; Related Information: Is Part Of SERS intership: Spring 1994 abstracts and research papers; Goldman, B.; PB: 195 p.
- Country of Publication:
- United States
- Language:
- English
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