ChIP-seq Mapping of Distant-Acting Enhancers and Their In Vivo Activities
The genomic location and function of most distant-acting transcriptional enhancers in the human genome remains unknown We performed ChIP-seq for various transcriptional coactivator proteins (such as p300) directly from different embryonic mouse tissues, identifying thousands of binding sitesTransgenic mouse experiments show that p300 and other co-activator peaks are highly predictive of genomic location AND tissue-specific activity patterns of distant-acting enhancersMost enhancers are active only in one or very few tissues Genomic location of tissue-specific p300 peaks correlates with tissue-specific expression of nearby genes Most binding sites are conserved, but the global degree of conservation varies between tissues
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- Genomics Division
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 1050657
- Report Number(s):
- LBNL-4785E-Poster; TRN: US201218%%869
- Resource Relation:
- Conference: Strategic BioSciences Review, Berkeley, CA, 4/26-27/2011
- Country of Publication:
- United States
- Language:
- English
Similar Records
ChIP-seq Accurately Predicts Tissue-Specific Activity of Enhancers
Tissue-Specific RNA Expression Marks Distant-Acting Developmental Enhancers