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Title: Hemicellulolytic organisms in the particle-associated microbiota of the hoatzin crop

Abstract

The hoatzin (Opisthocomus hoazin) is a South American herbivorous bird, that has an enlarged crop analogous to the rumen, where foregut microbes degrade the otherwise indigestible plant materials, providing energy to the host. The crop harbors an impressive array of microorganisms with potentially novel cellulolytic enzymes. Thie study describes the composition ofthe particle-associated microbiota in the hoatzin crop, combining a survey of 16S rRNA genes in 7 adult birds and metagenome sequencing of two animals. The pyrotag survey demonstrates that Prevotellaceae, are the most abundant and ubiquitous taxa, suggesting that the degradation of hemicellulose is an important activity in the crop. Nonetheless, preliminary results from the metagnome of the particle-associated microbiota of two adult birds show that the crop microbiome contains a high number of genes encoding cellulases (such as GH5) more abundant than those of the termite gut, as well as genes encoding hemicellulases. These preliminary results show that the carbohydate-active enzyme genes in the cropmetagenome could be a source of biochemical catalysts able to deconstruct plant biomass.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Genomics Division
OSTI Identifier:
1050654
Report Number(s):
LBNL-4787E-Poster
TRN: US201218%%866
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Conference
Resource Relation:
Conference: 6th Annual DOE JGI User Meeting , Walnut Creek, CA, 3/22-24
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; ADULTS; ANIMALS; BIOMASS; BIRDS; CATALYSTS; CELLULASE; CROPS; ENZYMES; GENES; HEMICELLULOSE; MICROORGANISMS; RUMINANTS; STOMACH; biochemical catalyst, hoatzin, plant biomass, cellulolytic enzymes

Citation Formats

Godoy-Vitorino, Filipa, Malfatti, Stephanie, Garcia-Amado, Maria A, Dominguez-Bello, Maria Gloria, Hugenholtz, Phillip, and Tringe, Susannah. Hemicellulolytic organisms in the particle-associated microbiota of the hoatzin crop. United States: N. p., 2011. Web.
Godoy-Vitorino, Filipa, Malfatti, Stephanie, Garcia-Amado, Maria A, Dominguez-Bello, Maria Gloria, Hugenholtz, Phillip, & Tringe, Susannah. Hemicellulolytic organisms in the particle-associated microbiota of the hoatzin crop. United States.
Godoy-Vitorino, Filipa, Malfatti, Stephanie, Garcia-Amado, Maria A, Dominguez-Bello, Maria Gloria, Hugenholtz, Phillip, and Tringe, Susannah. 2011. "Hemicellulolytic organisms in the particle-associated microbiota of the hoatzin crop". United States. https://www.osti.gov/servlets/purl/1050654.
@article{osti_1050654,
title = {Hemicellulolytic organisms in the particle-associated microbiota of the hoatzin crop},
author = {Godoy-Vitorino, Filipa and Malfatti, Stephanie and Garcia-Amado, Maria A and Dominguez-Bello, Maria Gloria and Hugenholtz, Phillip and Tringe, Susannah},
abstractNote = {The hoatzin (Opisthocomus hoazin) is a South American herbivorous bird, that has an enlarged crop analogous to the rumen, where foregut microbes degrade the otherwise indigestible plant materials, providing energy to the host. The crop harbors an impressive array of microorganisms with potentially novel cellulolytic enzymes. Thie study describes the composition ofthe particle-associated microbiota in the hoatzin crop, combining a survey of 16S rRNA genes in 7 adult birds and metagenome sequencing of two animals. The pyrotag survey demonstrates that Prevotellaceae, are the most abundant and ubiquitous taxa, suggesting that the degradation of hemicellulose is an important activity in the crop. Nonetheless, preliminary results from the metagnome of the particle-associated microbiota of two adult birds show that the crop microbiome contains a high number of genes encoding cellulases (such as GH5) more abundant than those of the termite gut, as well as genes encoding hemicellulases. These preliminary results show that the carbohydate-active enzyme genes in the cropmetagenome could be a source of biochemical catalysts able to deconstruct plant biomass.},
doi = {},
url = {https://www.osti.gov/biblio/1050654}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 31 00:00:00 EDT 2011},
month = {Tue May 31 00:00:00 EDT 2011}
}

Conference:
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