Engineered microorganisms capable of producing target compounds under anaerobic conditions
Abstract
The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
- Inventors:
-
- Denver, CO
- San Francisco, CA
- Parker, CO
- Irvine, CA
- Pasadena, CA
- La Canada, CA
- Publication Date:
- Research Org.:
- Gevo, Inc. (Englewood, CO)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1034914
- Patent Number(s):
- 8,097,440
- Application Number:
- 13/269,937
- Assignee:
- Gevo, Inc. (Englewood, CO); California Institute of Technology (Pasadena, CA)
- DOE Contract Number:
- FG02-07ER84893
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Buelter, Thomas, Meinhold, Peter, Feldman, Reid M. Renny, Hawkins, Andrew C, Urano, Jun, Bastian, Sabine, and Arnold, Frances. Engineered microorganisms capable of producing target compounds under anaerobic conditions. United States: N. p., 2012.
Web.
Buelter, Thomas, Meinhold, Peter, Feldman, Reid M. Renny, Hawkins, Andrew C, Urano, Jun, Bastian, Sabine, & Arnold, Frances. Engineered microorganisms capable of producing target compounds under anaerobic conditions. United States.
Buelter, Thomas, Meinhold, Peter, Feldman, Reid M. Renny, Hawkins, Andrew C, Urano, Jun, Bastian, Sabine, and Arnold, Frances. 2012.
"Engineered microorganisms capable of producing target compounds under anaerobic conditions". United States. https://www.osti.gov/servlets/purl/1034914.
@article{osti_1034914,
title = {Engineered microorganisms capable of producing target compounds under anaerobic conditions},
author = {Buelter, Thomas and Meinhold, Peter and Feldman, Reid M. Renny and Hawkins, Andrew C and Urano, Jun and Bastian, Sabine and Arnold, Frances},
abstractNote = {The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.},
doi = {},
url = {https://www.osti.gov/biblio/1034914},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 17 00:00:00 EST 2012},
month = {Tue Jan 17 00:00:00 EST 2012}
}
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