An assessment of PCB degradation by microogransims including methods for measuring mineralization

Technical Report · Mon Dec 31 00:00:00 EST 1990

DOI:https://doi.org/10.2172/10188676· OSTI ID:10188676

Hadden, C; Edenborn, H; Osborne, T; Holdsworth, G; Revis, N

These studies sought to isolate and identify organism(s) from PCB contaminated soil and sediment that degrade PCB; to provide information on the potential of organisms in soil samples taken from a PCB-contaminated area to mineralize or dechlorinate PCB congeners; to assess potential enhancement of PCB biodegradation as a result of nutritional amendment of the samples; and to carry out analyses of successive lysimeter samples to determine whether field treatments have had an effect on the capacity of soil microbes to mineralize PCBS. We have expended considerable effort to validate the fractionation procedure used to assess mineralization and conversion of PCB substrates. The assessment relies on the ability to measure [{sup 14}C]-labeled CO{sub 2} in the presence of potentially volatile [{sup 14}C]-labeled PCB and degradation products to differentiate between volatile and non-volatile [{sup 14}C]-labeled compounds between water-soluble products of metabolism and a mixture of unchanged substrate and other water-insoluble products and between metabolism and loss or non-extractability of the substrate.

• Research Organization: Oak Ridge Research Inst., TN (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC05-86OR21607
• OSTI ID: 10188676
• Report Number(s): DOE/OR/21607-T7; ON: DE94000861; BR: 820200000; TRN: 93:024020
• Resource Relation: Other Information: PBD: [1990]
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS
POLYCHLORINATED BIPHENYLS
BIODEGRADATION
MINERALIZATION
AEROBIC CONDITIONS
ANAEROBIC CONDITIONS
LAND RECLAMATION
SOILS
PESTICIDES
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
LYSIMETERS
540220
053000
540211
CHEMICALS MONITORING AND TRANSPORT
ENVIRONMENTAL ASPECTS
RADIOMETRIC TECHNIQUES