Role of x-ray-induced transcripts in adaptive responses following x-rays. Progress report, Year 2
I will describe our recent data in which we have extracted and purified a sufficient amount of RNA from primed and unprimed U1-Mel cells to begin the search for new genes which are modulated by priming or high dose irradiation during the establishment and/or challenge of adapted cells, respectively. Gene transcripts which are altered during ASRs now include alterations in xip5 (a gene with homology to human growth hormone), xipl2 (a gene with homology to human angiogenesis factor and a gene which may be involved in apoptosis due to its possible RNase activity), cyclin A (which is altered in primed cells only after a high dose of ionizing radiation), cyclin B (which is also altered in a similar manner as cyclin A), p53 (a tumor suppressor gene involved in cell division control in G{sub 1} following ionizing radiation), and glutathionine S transferase-pi (a gene product which has been demonstrated to be involved in DNA repair and redox cycling). In contrast, the remaining xip CDNA clones [i.e., xip1-4,6-11, which were isolated following high dose ionizing radiation exposure to human U1-Mel cells], Prad-1 (a gene involved in cell cycle controlling events at the G{sub 1} portion of the cell cycle), 36B4 (a gene involved in homeostasis), and cdc2 (a gene involved in the regulation of the S-phase portion of the cell cycle), were not altered following ionizing radiation, either during the establishment or challenge of adapted human cells.
- Research Organization:
- Michigan Univ., Ann Arbor, MI (United States). Dept. of Atmospheric and Oceanic Science
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-91ER61256
- OSTI ID:
- 10178681
- Report Number(s):
- DOE/ER/61256-T1; ON: DE93040079
- Resource Relation:
- Other Information: PBD: [1993]
- Country of Publication:
- United States
- Language:
- English
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