Large scale solubilization of coal and bioconversion to utilizable energy. Third quarterly technical progress report, April 1, 1994--June 30, 1994
In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, the investigators plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. They also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein. Main objectives are: (1) cloning of Neurospora gene for coal depolymerization protein controlling solubilization in different host cells, utilizing Neurospora plasmid and other vector(s); (2) (a) development of a large scale electrophoretic separation of coal drived products obtained after microbial solubilization; (b) identification of the coal derived products obtained after biosolubilization by Neurospora cultures or obtained after Neurospora enzyme catalyzed reaction in in vitro by the wildtype and mutant enzymes; (3) bioconversion of coal drived products into utilizable fuel; and (4) characterization of Neurospora wildtype and mutant CSA protein(s) involved in solubilization of coal in order to assess the nature of the mechanism of solubilization and the role of Neurospora proteins in this process.
- Research Organization:
- South Carolina Univ., Columbia, SC (United States). Dept. of Biological Sciences
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG22-93PC93224
- OSTI ID:
- 10176781
- Report Number(s):
- DOE/PC/93224-T3; ON: DE94017551; BR: AA1525050
- Resource Relation:
- Other Information: PBD: [1994]
- Country of Publication:
- United States
- Language:
- English
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