Detection of single molecules in microspheres

Conference · Thu Jul 01 00:00:00 EDT 1993

OSTI ID:10176052

Barnes, M D; Whitten, W B; Ramsey, J M ^[1]; Ng, K C ^[2]; Arnold, S ^[3]

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1. Oak Ridge National Lab., TN (United States)
2. California State Univ., Fresno, CA (United States). Dept. of Chemistry
3. Polytechnic Inst. of Brooklyn, NY (United States). Microparticle Photophysics Lab.

We have investigated the use of micron-sized liquid droplets as sample medium to detect single fluorescent molecules in solution. The use of microdroplets (5--15 {mu}m diameter) offers several powerful advantages over single-molecule detection schemes involving measurements on bulk liquids where the probe volume is defined by the laser beam. In addition, cavity-quantum electrodynamical (QED) effects have been observed which influence both spontaneous emission rates and fluorescence yields of dye molecules in these microspheres.

• Research Organization: Oak Ridge National Lab., TN (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 10176052
• Report Number(s): CONF-930159-53; ON: DE93017776
• Resource Relation: Conference: OE/LASE `93: International Society for Optical Engineering (SPIE) conference,Los Angeles, CA (United States),13-23 Jan 1993; Other Information: PBD: [1993]
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
MOLECULES
DETECTION
DYES
LIQUIDS
DROPLETS
MICROSPHERES
FLUORESCENCE
MICROANALYSIS
SOLUTIONS
LASER RADIATION
LEVITATION
400102
CHEMICAL AND SPECTRAL PROCEDURES