Phylogenetic relationships among subsurface microorganisms. Project technical progress report
The development of group-specific, 16S ribosomal RNA-targeted oligonucleotide hybridization probes for the rapid detection of specific types of subsurface microorganisms is described. Because portions of the 16S RRNA molecule are unique to particular organisms or groups, these unique sequences can serve as targets for hybridization probes with varied specificity. Target sequences for selected microbial groups have been identified by analysis of the available RRNA sequence data for subsurface microbes. Hybridization probes for these target sequences were produced and their effectiveness and specificity tested with RNA cell blot and in situ hybridizations. Selected probes were used to study phylogenetic relationships among subsurface microbes and to classify these organisms into the specific groups that the probes are designed to detect. To date, this work has been performed on the P24 and C10 borehole isolates from the Savannah River Site. The probes will also be used, with in situ hybridizations, to detect and monitor selected microbial groups in freshly collected subsurface samples and laboratory microcosms in collaboration with other investigators. In situ hybridizations permit detection of selected microbial types without the necessity to isolate and culture them in the laboratory.
- Research Organization:
- Rensselaer Polytechnic Inst., Troy, NY (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-90ER60989
- OSTI ID:
- 10171574
- Report Number(s):
- DOE/ER/60989-3; ON: DE93018323
- Resource Relation:
- Other Information: PBD: [1993]
- Country of Publication:
- United States
- Language:
- English
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Phylogenetic relationships among subsurface microorganisms. Progress report
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