The use of dimorphic Alu insertions in human DNA fingerprinting
- Florida International Univ., Miami, FL (United States). Dept. of Biological Sciences
- Lawrence Livermore National Lab., CA (United States)
- Louisiana State Univ., New Orleans, LA (United States). Medical Center
We have characterized certain Human Specific Alu Insertions as either dimorphic (TPA25, PV92, APO), sightly dimorphic (C2N4 and C4N4) or monomorphic (C3N1, C4N6, C4N2, C4N5, C4N8), based on studies of Caucasian, Asian, American Black and African Black populations. Our approach is based upon: (1) PCR amplification using primers directed to the sequences that flank the site of insertion of the different Alu elements studied; (2) gel electrophoresis and scoring according to the presence or absence of an Alu insertion in one or both homologous chromosomes; (3) allelic frequencies calculated and compared according to Hardy-Weinberg equilibrium. Our DNA fingerprinting procedure using PCR amplification of dimorphic Human Specific Alu insertions, is stable enough to be used not only as a tool for genetic mapping but also to characterize populations, study migrational patterns and track the inheritance of human genetic disorders.
- Research Organization:
- Lawrence Livermore National Lab., CA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 10170149
- Report Number(s):
- UCRL-JC-112318; CONF-9211249-1; ON: DE93016530
- Resource Relation:
- Conference: 2. international symposium on DNA fingerprinting,Belo Horizonte (Brazil),9 Nov 1992; Other Information: PBD: 4 Dec 1992
- Country of Publication:
- United States
- Language:
- English
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