Multiplexed lateral flow microarray assay for detection of citrus pathogens Xylella fastidiosa and Xanthomonas axonopodis pv citri

Cary, R. Bruce; Stubben, Christopher J.

Title: Multiplexed lateral flow microarray assay for detection of citrus pathogens Xylella fastidiosa and Xanthomonas axonopodis pv citri

Patent · Tue Mar 22 00:00:00 EDT 2011

OSTI ID:1016352

Cary, R. Bruce ^[1]; Stubben, Christopher J. ^[2]

Santa Fe, NM
Los Alamos, NM

The invention provides highly sensitive and specific assays for the major citrus pathogens Xylella fastidiosa and Xanthomonas axonopodis, including a field deployable multiplexed assay capable of rapidly assaying for both pathogens simultaneously. The assays are directed at particular gene targets derived from pathogenic strains that specifically cause the major citrus diseases of citrus variegated chlorosis (Xylella fastidiosa 9a5c) and citrus canker (Xanthomonas axonopodis pv citri). The citrus pathogen assays of the invention offer femtomole sensitivity, excellent linear dynamic range, and rapid and specific detection.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-06NA25396

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Number(s):: 7,910,309

Application Number:: US Patent Application 12/221,351

OSTI ID:: 1016352

Country of Publication:: United States

Language:: English

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