Illumina Unamplified Indexed Library Construction: An Automated Approach
Conference
·
OSTI ID:1015999
Manual library construction is a limiting factor in Illumina sequencing. Constructing libraries by hand is costly, time-consuming, low-throughput, and ergonomically hazardous, and constructing multiple libraries introduces risk of library failure due to pipetting errors. The ability to construct multiple libraries simultaneously in automated fashion represents significant cost and time savings. Here we present a strategy to construct up to 96 unamplified indexed libraries using Illumina TruSeq reagents and a Biomek FX robotic platform. We also present data to indicate that this library construction method has little or no risk of cross-contamination between samples.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- Genomics Division
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 1015999
- Report Number(s):
- LBNL-4521E-Poster; TRN: US201113%%124
- Resource Relation:
- Conference: DOE JGI User Meeting
- Country of Publication:
- United States
- Language:
- English
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