Molecular biological enhancement of coal biodesulfurization. Fourth quarterly technical progress report

Technical Report · Thu Jun 14 00:00:00 EDT 1990

DOI:https://doi.org/10.2172/10159542· OSTI ID:10159542

Litchfield, J H; Fry, I; Wyza, R E; Palmer, D T; Zupancic, T J; Conkle, H N

The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the ``4S`` pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; transfer this pathway into a fast-growing chemolithotropic bacterium; conduct a batch-mode optimization/analysis of scale-up variables.

• Research Organization: Battelle Memorial Inst., Columbus, OH (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC22-89PC89902
• OSTI ID: 10159542
• Report Number(s): DOE/PC/89902-T10; ON: DE92015657
• Resource Relation: Other Information: PBD: 14 Jun 1990
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
59 BASIC BIOLOGICAL SCIENCES
COAL
DESULFURIZATION
ORGANIC SULFUR COMPOUNDS
BIODEGRADATION
MICROORGANISMS
POLYCYCLIC SULFUR HETEROCYCLES
QUANTITATIVE CHEMICAL ANALYSIS
FLUORESCENCE
CULTURE MEDIA
CHROMATOGRAPHY
DNA
CLONING
GENETIC ENGINEERING
ENZYMES
SULFOXIDES
SULFONES
SULFONATES
SULFATES
PSEUDOMONAS
BACILLUS
PROGRESS REPORT
010402
550700
550400
PURIFICATION AND UPGRADING
MICROBIOLOGY
GENETICS