Role of proteolytic enzymes in degradation of plant tissues. Summary of results of studies completed on the prior
Strain SR 394 of Erwinia carotovora (Ecc) produced proteases constitutively in all media tested. Growth of Ecc and production of protease were enhanced significantly by the presence of poetic materials and/or plant call walls in the test media. After electrofocusing, one major and one minor protease bands, at PI 4.8 and PI 5.1, respectively, were detected. Only one band of 43 kDa was detected on SDS gels. Only one protease band was detected in SDS gels of infected plant extracts. This protease was purified to homogeneity. It in a highly thermostable metal protease; it degrades gelatin, soluble collagen and hide powderazure, shows weak activity on casein and azocasein, but does not degrade insoluble collagen or elastin.
- Research Organization:
- Wisconsin Univ., Madison, WI (United States). Dept. of Plant Pathology
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-87ER13717
- OSTI ID:
- 10158249
- Report Number(s):
- DOE/ER/13717-3; ON: DE93015740
- Resource Relation:
- Other Information: PBD: [1991]
- Country of Publication:
- United States
- Language:
- English
Similar Records
The role of proteolytic enzymes in degradation of plant tissues: Summary report
[Induction of polygalacturonases important in pathogenicity of Pseudomonas solanacearum]