An improved method for producing radiation hybrids applied to human chromosome 19. Technical progress report, 1 March 1993--28 February 1994

Technical Report · Sun May 01 00:00:00 EDT 1994

DOI:https://doi.org/10.2172/10156883· OSTI ID:10156883

Jackson, C L; Mark, H F.L.

Markers for which hybrids have been characterized have doubled from approximately 25 to 50 markers. Almost all new markers are microsatellite dinucleotide repeat markers. This will allow correlation of fragments of chromosome 19 in the hybrids with the genetic map. In situ hybridization is certainly one of the most direct methods of gene mapping in general and the most direct method to visualize the number of chromosome fragments in a hybrid. We have utilized fluorescent in situ hybridization (FISH) to determine the number of human fragments contained in the radiation hybrid cell lines.

• Research Organization: Rhode Island Hospital, Providence, RI (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER61136
• OSTI ID: 10156883
• Report Number(s): DOE/ER/61136-3; ON: DE94011572; TRN: 94:006016
• Resource Relation: Other Information: PBD: [1994]
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 19
DNA HYBRIDIZATION
GENETIC MAPPING
BIOLOGICAL MARKERS
PROGRESS REPORT
CHROMOSOMAL ABERRATIONS
550200
550400
BIOCHEMISTRY
GENETICS