The chlorophyll-binding protein CP47 in photosystem II. Progress report
The CP47 protein is encoded by the psbB gene, binds 10-20 chlorophyll and has an apparent molecular weight of about 47 kDa. Specific mutations can be introduced into this protein using the transformable, (photo)heterotrophic cyanobacterium Synechocystis sp. PCC 6803. This cyanobacterium takes up foreign DNA spontaneously, and can survive in the absence of PS II activity to take out the wild-type psbB gene and subsequently replace it by a construct containing the desired mutation(s) in psbB. The PS I-less Synechocystis system we are now utilizing for studies of His mutations in the CP47 protein is a very useful strain for detailed studies of pigment-protein interactions and of the effects of limited pheophytinization in the antenna on light-harvesting efficiency. This is providing new information on parameters influencing energy transfer efficiency in photosynthetic systems. Moreover, studies regarding the hydrophilic regions of the CP47 protein are contributing to an understanding of the role of this protein in optimizing water-splitting activity.
- Research Organization:
- Arizona State Univ., Tempe, AZ (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-89ER14031
- OSTI ID:
- 10155644
- Report Number(s):
- DOE/ER/14031-4; ON: DE94012837; BR: KC0600000
- Resource Relation:
- Other Information: PBD: [1994]
- Country of Publication:
- United States
- Language:
- English
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