Effects of low-dose radiation on gene expression in Syrian hamster embryo cells: Comparison of JANUS neutrons and gamma rays
Past work by or group and others has shown the modulation of specific genes following exposure of cells to ionizing radiation. Many classes of genes have been found to be modulated in response to ionizing radiation, including those encoding cytoskeletal elements, cell growth arresting proteins, cytokines, and cellular oncogenes. The functions of this specific modulation of gene expression are currently being investigated by several groups: it has been suggested that gene modulation in response to radiation plays a role in the cellular repair of DNA damage, cell survival, or cellular transformation. Several groups have examined induction of nuclear proto-oncogenes following exposure to DNA-damaging agents. In all experiments, we examined modulation of gene expression by ionizing radiations in Syrian hamster embryo (SHE) fibroblasts, which are normal diploid cells that can be neoplastically transformed by low doses of ionizing radiations. Cells plated in 100-mm Petri plates containing 10 ml of medium were irradiated with {sup 60}C {gamma}-rays or fission-spectrum neutrons (0.85 MeV) from the JANUS reactor. All irradiations were performed at 37{degrees}C on cycling cells; equitoxic doses of neutrons and {gamma}-rays were selected on the basis of survival data.
- Research Organization:
- Argonne National Lab., IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 10155018
- Report Number(s):
- ANL/CP-75218; CONF-920720-1; ON: DE92016176
- Resource Relation:
- Conference: International conference on low dose irradiation and biological defense mechanisms,Kyoto (Japan),12-16 Jul 1992; Other Information: PBD: [1992]
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
HAMSTERS
GENETIC RADIATION EFFECTS
EMBRYOS
IONIZING RADIATIONS
DOSE RATES
PROTEINS
RNA
DNA
560120
560150
550400
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
RADIATION EFFECTS ON ANIMALS
GENETICS