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Title: Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5. Final report

Abstract

The object of our current grant is to develop novel methods for mapping of the human genome. The techniques to be assessed were: (1) three methods for the production of unique sequence clones from the region of interest; (2) novel methods for the production and separation of multi-megabase DNA fragments; (3) methods for the production of ``physical linking clones`` that contain rare restriction sites; (4) application of these methods and available resources to map the region of interest. Progress includes: In the first two years methods were developed for physical mapping and the production of arrayed clones; We have concentrated on developing rare- cleavage tools based or restriction endonucleases and methylases; We studied the effect of methylation on enzymes used for PFE mapping of the human genome; we characterized two new isoschizomers of rare cutting endonucleases; we developed a reliable way to produce partial digests of DNA in agarose plugs and applied it to the human genome; and we applied a method to double the apparent specificity of the ``rare-cutter`` endonucleases.

Authors:
Publication Date:
Research Org.:
California Inst. of Biological Research, La Jolla, CA (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
10154158
Report Number(s):
DOE/ER/60913-4
ON: DE92016055
DOE Contract Number:  
FG03-89ER60913
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: Dec 1991
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN CHROMOSOME 5; GENETIC MAPPING; OPTIMIZATION; ENDONUCLEASES; BIOCHEMICAL REACTION KINETICS; DNA METHYLASES; ENZYME ACTIVITY; PROGRESS REPORT; 550400; 550200; GENETICS; BIOCHEMISTRY

Citation Formats

McClelland, M. Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5. Final report. United States: N. p., 1991. Web. doi:10.2172/10154158.
McClelland, M. Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5. Final report. United States. https://doi.org/10.2172/10154158
McClelland, M. 1991. "Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5. Final report". United States. https://doi.org/10.2172/10154158. https://www.osti.gov/servlets/purl/10154158.
@article{osti_10154158,
title = {Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5. Final report},
author = {McClelland, M},
abstractNote = {The object of our current grant is to develop novel methods for mapping of the human genome. The techniques to be assessed were: (1) three methods for the production of unique sequence clones from the region of interest; (2) novel methods for the production and separation of multi-megabase DNA fragments; (3) methods for the production of ``physical linking clones`` that contain rare restriction sites; (4) application of these methods and available resources to map the region of interest. Progress includes: In the first two years methods were developed for physical mapping and the production of arrayed clones; We have concentrated on developing rare- cleavage tools based or restriction endonucleases and methylases; We studied the effect of methylation on enzymes used for PFE mapping of the human genome; we characterized two new isoschizomers of rare cutting endonucleases; we developed a reliable way to produce partial digests of DNA in agarose plugs and applied it to the human genome; and we applied a method to double the apparent specificity of the ``rare-cutter`` endonucleases.},
doi = {10.2172/10154158},
url = {https://www.osti.gov/biblio/10154158}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Dec 01 00:00:00 EST 1991},
month = {Sun Dec 01 00:00:00 EST 1991}
}