Microbial strain improvement for organosulfur removal from coal. Technical report, December 1, 1992--February 28, 1993
- Institute of Gas Technology, Chicago, IL (United States)
- Illinois Clean Coal Inst., Carterville, IL (United States)
IGT has developed a microbial culture of Rhodococcus rhodochrous, designated as IGTS8, that is capable of specifically cleaving carbon-sulfur bonds in a range of organosulfur model compounds and is capable of removing organic sulfur from coal and petroleum without significantly sacrificing the calorific value of the fuel. Although IGTSS possesses the ability to specifically remove organic sulfur from coal, a major research need is to develop strains of microorganisms that possess higher levels of desulfurization activity. During the past quarter, the DNA sequence of the promoter region of the chloramphenicol resistance gene of pRF2 was determined and tentatively localized to a 50 bp region. To further define the important sequences of this promoter mutants were generated that may have altered promoters allowing higher levels of expression of the chloramphenicol resistance gene. The promoter region from twenty such mutants has been subcloned. Additionally, promoter probe vectors were used to isolate small DNA fragments from the chromosome that possess promoters. These studies will lead to the identification and/or the construction of particularly strong Rhodococcus promoters which will subsequently be used to express the desulfurization genes.
- Research Organization:
- Illinois Dept. of Energy and Natural Resources, Springfield, IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FC22-92PC92521
- OSTI ID:
- 10149989
- Report Number(s):
- DOE/PC/92521-T19; ON: DE93013638
- Resource Relation:
- Other Information: PBD: [1993]
- Country of Publication:
- United States
- Language:
- English
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